Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chung-Chieh Yu
  • James W. Tunnell
  • Martin Hunter
  • Maxim Kalashnikov
  • Christopher Fang-Yen
  • Stephen F. Fulghum
  • Kamran Badizadegan
  • Ramachandra R. Dasari
  • Michael S. Feld

Detail(s)

Original languageEnglish
Pages (from-to)3119-3121
Journal / PublicationOptics Letters
Volume31
Issue number21
Publication statusPublished - 1 Nov 2006
Externally publishedYes

Abstract

We describe azimuthal light scattering spectroscopy (φ/LSS), a novel technique for assessing epithelial-cell nuclear morphology. The difference between the spectra measured at azimuthal angles φ=0° and φ=90° preferentially isolates the single backscattering contribution due to large (∼10 μm) structures such as epithelial cell nuclei by discriminating against scattering from smaller organelles and diffusive background. We demonstrate the feasibility of using φ/LSS for cancer detection by showing that spectra from cancerous colon tissue exhibit significantly greater azimuthal asymmetry than spectra from normal colonic tissues. © 2006 Optical Society of America.

Citation Format(s)

Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy. / Yu, Chung-Chieh; Lau, Condon; Tunnell, James W. et al.
In: Optics Letters, Vol. 31, No. 21, 01.11.2006, p. 3119-3121.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review