Ars2 Links the Nuclear Cap-Binding Complex to RNA Interference and Cell Proliferation

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Joshua J. Gruber
  • D. Steven Zatechka
  • Leah R. Sabin
  • Jeongsik Yong
  • Julian J. Lum
  • Mei Kong
  • Wei-Xing Zong
  • Zhenxi Zhang
  • Jason Rawlings
  • Sara Cherry
  • James N. Ihle
  • Gideon Dreyfuss
  • Craig B. Thompson

Detail(s)

Original languageEnglish
Pages (from-to)328-339
Journal / PublicationCell
Volume138
Issue number2
Publication statusPublished - 23 Jul 2009
Externally publishedYes

Abstract

Here we identify a component of the nuclear RNA cap-binding complex (CBC), Ars2, that is important for miRNA biogenesis and critical for cell proliferation. Unlike other components of the CBC, Ars2 expression is linked to the proliferative state of the cell. Deletion of Ars2 is developmentally lethal, and deletion in adult mice led to bone marrow failure whereas parenchymal organs composed of nonproliferating cells were unaffected. Depletion of Ars2 or CBP80 from proliferating cells impaired miRNA-mediated repression and led to alterations in primary miRNA processing in the nucleus. Ars2 depletion also reduced the levels of several miRNAs, including miR-21, let-7, and miR-155, that are implicated in cellular transformation. These findings provide evidence for a role for Ars2 in RNA interference regulation during cell proliferation. © 2009 Elsevier Inc. All rights reserved.

Research Area(s)

  • CELLCYCLE, RNA

Citation Format(s)

Ars2 Links the Nuclear Cap-Binding Complex to RNA Interference and Cell Proliferation. / Gruber, Joshua J.; Zatechka, D. Steven; Sabin, Leah R.; Yong, Jeongsik; Lum, Julian J.; Kong, Mei; Zong, Wei-Xing; Zhang, Zhenxi; Lau, Chi-Kong; Rawlings, Jason; Cherry, Sara; Ihle, James N.; Dreyfuss, Gideon; Thompson, Craig B.

In: Cell, Vol. 138, No. 2, 23.07.2009, p. 328-339.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review