@inbook{a18a44d768fc492080202e2c71cce0f7,
title = "Aptamers as Functional Modules for DNA Nanostructures",
abstract = "Watson-Crick base-pairing of DNA allows the nanoscale fabrication of biocompatible synthetic nanostructures for diagnostic and therapeutic biomedical purposes. DNA nanostructure design elicits exquisite control of shape and conformation compared to other nanoparticles. Furthermore, nucleic acid aptamers can be coupled to DNA nanostructures to allow interaction and response to a plethora of biomolecules beyond nucleic acids. When compared to the better-known approach of using protein antibodies for molecular recognition, nucleic acid aptamers are bespoke with the underlying DNA nanostructure backbone and have various other stability, synthesis, and cost advantages. Here, we provide detailed methodologies to synthesize and characterize aptamer-enabled DNA nanostructures. The methods described can be generally applied to various designs of aptamer-enabled DNA nanostructures with a wide range of applications both within and beyond biomedical nanotechnology. {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2023.",
keywords = "Aptamers, Atomic force microscopy, Bioanalytical sensors, Biophysical assays, Circular dichroism, DNA nanostructures, Droplet microfluidic SELEX, Transmission electron microscopy",
author = "Shiu, \{Simon Chi-Chin\} and Kinghorn, \{Andrew B.\} and Wei Guo and Slaughter, \{Liane S.\} and Danyang Ji and Xiaoyong Mo and Lin Wang and Tran, \{Ngoc Chau\} and Kwok, \{Chun Kit\} and Shum, \{Anderson Ho Cheung\} and Tse, \{Edmund Chun Ming\} and Tanner, \{Julian A.\}",
year = "2023",
doi = "10.1007/978-1-0716-3028-0\_17",
language = "English",
isbn = "978-1-0716-3027-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "301--337",
editor = "Juli{\'a}n Valero",
booktitle = "DNA and RNA Origami",
address = "United States",
}