Aptamers as Functional Modules for DNA Nanostructures
Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 12 - Chapter in an edited book (Author) › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
| Original language | English |
|---|---|
| Title of host publication | DNA and RNA Origami |
| Subtitle of host publication | Methods and Protocols |
| Editors | Julián Valero |
| Place of Publication | New York, NY |
| Publisher | Humana Press |
| Pages | 301-337 |
| ISBN (electronic) | 978-1-0716-3028-0 |
| ISBN (print) | 978-1-0716-3027-3, 978-1-0716-3030-3 |
| Publication status | Published - 2023 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2639 |
| ISSN (Print) | 1064-3745 |
| ISSN (electronic) | 1940-6029 |
Link(s)
Abstract
Watson-Crick base-pairing of DNA allows the nanoscale fabrication of biocompatible synthetic nanostructures for diagnostic and therapeutic biomedical purposes. DNA nanostructure design elicits exquisite control of shape and conformation compared to other nanoparticles. Furthermore, nucleic acid aptamers can be coupled to DNA nanostructures to allow interaction and response to a plethora of biomolecules beyond nucleic acids. When compared to the better-known approach of using protein antibodies for molecular recognition, nucleic acid aptamers are bespoke with the underlying DNA nanostructure backbone and have various other stability, synthesis, and cost advantages. Here, we provide detailed methodologies to synthesize and characterize aptamer-enabled DNA nanostructures. The methods described can be generally applied to various designs of aptamer-enabled DNA nanostructures with a wide range of applications both within and beyond biomedical nanotechnology. © Springer Science+Business Media, LLC, part of Springer Nature 2023.
Research Area(s)
- Aptamers, Atomic force microscopy, Bioanalytical sensors, Biophysical assays, Circular dichroism, DNA nanostructures, Droplet microfluidic SELEX, Transmission electron microscopy
Citation Format(s)
Aptamers as Functional Modules for DNA Nanostructures. / Shiu, Simon Chi-Chin; Kinghorn, Andrew B.; Guo, Wei et al.
DNA and RNA Origami: Methods and Protocols. ed. / Julián Valero. New York, NY: Humana Press, 2023. p. 301-337 (Methods in Molecular Biology; Vol. 2639).
DNA and RNA Origami: Methods and Protocols. ed. / Julián Valero. New York, NY: Humana Press, 2023. p. 301-337 (Methods in Molecular Biology; Vol. 2639).
Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 12 - Chapter in an edited book (Author) › peer-review
