Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions

Suihong Huang; Qingqing Lu; Ming Ho Choi; Xuebing Zhang; Jin Young Kim

doi:10.1016/j.xpro.2021.100416

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Related Research Unit(s)

Detail(s)

Original language	English
Article number	100416
Journal / Publication	STAR Protocols
Volume	2
Issue number	2
Online published	31 Mar 2021
Publication status	Published - 18 Jun 2021

Link(s)

DOI	DOI https://doi.org/10.1016/j.xpro.2021.100416 Final Published version
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Attachment(s)	Documents 74470358.pdf Final Published version, 3.89 MB, PDF Publisher's Copyright Statement This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85103400432&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(1e4f06a1-5d14-48c6-9533-5e67bebc0322).html

Abstract

This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain.
For complete details on the use and execution of this protocol, please refer to Huang et al. (2020).