Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions

Suihong Huang; Qingqing Lu; Ming Ho Choi; Xuebing Zhang; Jin Young Kim

doi:10.1016/j.xpro.2021.100416

Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions

Suihong Huang, Qingqing Lu, Ming Ho Choi, Xuebing Zhang, Jin Young Kim^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

3 Citations (Scopus)

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Abstract

This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain.
For complete details on the use and execution of this protocol, please refer to Huang et al. (2020).

Original language	English
Article number	100416
Journal	STAR Protocols
Volume	2
Issue number	2
Online published	31 Mar 2021
DOIs	https://doi.org/10.1016/j.xpro.2021.100416
Publication status	Published - 18 Jun 2021

Research Keywords

Cell Biology
Model Organisms
Neuroscience

Publisher's Copyright Statement

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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10.1016/j.xpro.2021.100416

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title = "Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions",

abstract = "This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain. For complete details on the use and execution of this protocol, please refer to Huang et al. (2020).",

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AU - Huang, Suihong

AU - Lu, Qingqing

AU - Choi, Ming Ho

AU - Zhang, Xuebing

AU - Kim, Jin Young

PY - 2021/6/18

Y1 - 2021/6/18

N2 - This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain. For complete details on the use and execution of this protocol, please refer to Huang et al. (2020).

AB - This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain. For complete details on the use and execution of this protocol, please refer to Huang et al. (2020).

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KW - Model Organisms

KW - Neuroscience

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DO - 10.1016/j.xpro.2021.100416

M3 - RGC 21 - Publication in refereed journal

C2 - 33870223

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 100416

ER -

Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions

Abstract

Research Keywords

Publisher's Copyright Statement

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GRF: The Circadian Clock-dependent Cell-fate Decision of Adult Neural Stem Cells: How the Circadian Clock Controls Demyelination/Remyelination in the Brain

GRF: Communication Mechanisms between Transcriptional and Translational Machineries through the Circadian Clock

ECS: New Insight into HDAC1 as a Molecular Linker between Neurodegeneration and Circadian Clocks

Cite this

Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions

Abstract

Research Keywords

Publisher's Copyright Statement

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

GRF: The Circadian Clock-dependent Cell-fate Decision of Adult Neural Stem Cells: How the Circadian Clock Controls Demyelination/Remyelination in the Brain

GRF: Communication Mechanisms between Transcriptional and Translational Machineries through the Circadian Clock

ECS: New Insight into HDAC1 as a Molecular Linker between Neurodegeneration and Circadian Clocks

Cite this