Application of CRISPR/Cas9-Based Genome Editing in Studying the Mechanism of Pandrug Resistance in Klebsiella pneumoniae

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Qiaoling Sun
  • Yu Wang
  • Ning Dong
  • Lingwei Shen
  • Hongwei Zhou
  • Yanyan Hu
  • Danxia Gu
  • Rong Zhang
  • Quanjiang Ji

Detail(s)

Original languageEnglish
Article numbere00113-19
Journal / PublicationAntimicrobial Agents and Chemotherapy
Volume63
Issue number7
Online published24 Jun 2019
Publication statusPublished - Jul 2019
Externally publishedYes

Abstract

In this study, a CRISPR/Cas9-mediated genome editing method was used to study the functions of the mgrB, tetA, and ramR genes in mediating colistin and tigecycline resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP). Inactivation of the tetA or ramR gene or the mgrB gene by CRISPR/Cas9 affected bacterial susceptibility to tigecycline or colistin, respectively. This study proved that the CRISPR/Cas9-based genome editing method could be effectively applied to K. pneumoniae and should be further utilized for genetic characterization.

Research Area(s)

  • CRISPR/Cas9, Drug resistance mechanisms, Genome editing, Klebsiella pneumoniae, Pandrug resistance

Citation Format(s)

Application of CRISPR/Cas9-Based Genome Editing in Studying the Mechanism of Pandrug Resistance in Klebsiella pneumoniae. / Sun, Qiaoling; Wang, Yu; Dong, Ning; Shen, Lingwei; Zhou, Hongwei; Hu, Yanyan; Gu, Danxia; Chen, Sheng; Zhang, Rong; Ji, Quanjiang.

In: Antimicrobial Agents and Chemotherapy, Vol. 63, No. 7, e00113-19, 07.2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal