Anti-fouling magnetic nanoparticles for siRNA delivery

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Cyrille Boyer
  • Priyanto Priyanto
  • Thomas P. Davis
  • Dakrong Pissuwan
  • Volga Bulmus
  • Maria Kavallaris
  • Rose Amal
  • Matt Carroll
  • Robert Woodward
  • Tim St Pierre

Detail(s)

Original languageEnglish
Pages (from-to)255-265
Journal / PublicationJournal of Materials Chemistry
Volume20
Issue number2
Publication statusPublished - 2010
Externally publishedYes

Abstract

Iron oxide nanoparticles (IONPs), with a diameter of 8 nm, have been coated with two different polymers, i.e. poly(oligoethylene glycol) methyl ether acrylate (P(OEG-A)) and poly(dimethylaminoethyl acrylate) (P(DMAEA)). The polymers were attached to the nanoparticle surface using two different strategies, with the aim of creating an internal layer of P(DMAEA) and an outer shell of P(OEG-A). The subsequent polymer-stabilized IONPs were characterized using ATR, XPS and TGA, proving the presence of polymers on the IONP surfaces with a grafting density ranging from 0.05 to 0.22 chain per nm2. High grafting densities were demonstrated when the two homopolymers were assembled on the surfaces of the IONPs simultaneously. The polymer composition at the surfaces of the IONPs could be controlled by manipulating the feed ratio P(OEG-A)-P(DMAEA) present in solution. These hybrid organic-inorganic particles (70-150 nm) proved to be stable in both water and 50 vol% fetal bovine serum (FBS). In addition, zeta-potential measurements confirmed that P(OEG-A) chains effectively mask the positive charge originating from P(DMAEA) thereby limiting protein adsorption on these particles. Hybrid nanoparticles were exploited for the complexation of siRNA, thereby generating IONP siRNA nano-carriers with anti-fouling P(OEG-A) shells. The transfection efficiency was measured using human neuroblastoma SHEP cells both in the presence and in the absence of a magnetic field in FBS. The transfection efficiency was determined by both fluorescence microscopy and flow cytometry. Cytotoxicity studies revealed that the IONP carriers were non-toxic to SHEP cells. In addition, studies on the proton transverse relaxation enhancement properties of these stabilized IONPs indicated high relaxivities (∼160 s-1 per mM of Fe). © 2010 The Royal Society of Chemistry.

Citation Format(s)

Anti-fouling magnetic nanoparticles for siRNA delivery. / Boyer, Cyrille; Priyanto, Priyanto; Davis, Thomas P. et al.
In: Journal of Materials Chemistry, Vol. 20, No. 2, 2010, p. 255-265.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review