Anti-angiogenic activity of heparin functionalised cerium oxide nanoparticles

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Megan S. Lord
  • Bonny Tsoi
  • Cindy Gunawan
  • Rose Amal
  • John M. Whitelock

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)8808-8818
Journal / PublicationBiomaterials
Volume34
Issue number34
Online published12 Aug 2013
Publication statusPublished - Nov 2013

Abstract

Cerium oxide nanoparticles (nanoceria) are widely reported to be non-cytotoxic and modulate intracellular reactive oxygen species (ROS). In this study, nanoceria (dxRD=12nm) were functionalised witheither 130 or 880 molecules of unfractionated heparin using the organosilane linker, 3-aminopropyltriethoxysilane. Nanoceria with a low level of heparin functionalisation were found to scavenge intracellular ROS to the same extent as unfunctionalised nanoceria and significantly more than cells exposed to medium only. In contrast, nanoceria with the highest level of heparin functionalisation were not as effective at scavenging intracellular ROS. Nanoceria were localised predominantly in the cytoplasm, while heparin-nanoceria were localised in both the cytoplasm and lysosomes. Together these data demonstrated that the level of nanoceria surface functionalisation with heparin determined the intracellular localisation and ROS scavenging ability of these particles. Additionally, heparin-nanoceria were effective in reducing endothelial cell proliferation indicating that they may find application in the control of angiogenesis in cancer in the future. © 2013 Elsevier Ltd.

Research Area(s)

  • Angiogenesis, Cerium oxide, Endothelial cells, Heparin, Nanoparticle, Reactive oxygen species

Citation Format(s)

Anti-angiogenic activity of heparin functionalised cerium oxide nanoparticles. / Lord, Megan S.; Tsoi, Bonny; Gunawan, Cindy; Teoh, Wey Yang; Amal, Rose; Whitelock, John M.

In: Biomaterials, Vol. 34, No. 34, 11.2013, p. 8808-8818.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review