Annexin V-containing cubosomes for targeted early detection of apoptosis in degenerative retinal tissue

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Author(s)

  • Yue Ding
  • Seong Hoong Chow
  • Guei-Sheung Liu
  • Bo Wang
  • Tsung-Wu Lin
  • Anthony P. Duff
  • Anton P. Le Brun
  • Hsin-Hui Shen

Detail(s)

Original languageEnglish
Pages (from-to)7652-7661
Journal / PublicationJournal of Materials Chemistry B
Volume6
Issue number46
Early online date26 Oct 2018
Publication statusPublished - 14 Dec 2018

Abstract

New drug delivery materials targeting damaged ocular tissues are of particular interest. In this work, we have formulated annexin/phosphatidylserine/phytantriol and annexin/phosphatidylserine/monoolein cubosomes based on incorporation of 1,2-dipalmitoyl-sn-glycero-3-phospho-L-serine (PS) lipid and annexin V (ANX) protein with phytantriol (Phy) and monoolein (MO) respectively. The incorporation of ANX is important because it can be used as a diagnostic tool for in vivo apoptosis detection due to its high affinity to phosphatidylserine in the presence of Ca2+. We have also prepared PS-Phy and PS-MO cubosomes without ANX as a comparison, and characterized them using dynamic light scattering, cryo-TEM images and small-angle X-ray scattering, showing that PS-Phy cubosomes have greater chemical stability, and that ANX-PS-Phy cubosomes have the potential for in vivo drug delivery. In addition, we have reconstituted an apoptotic biomimetic membrane on a surface to gain insights into cubosome-bilayer interactions using a quartz-crystal microbalance and neutron reflectometry. The neutron reflectivity data reveal that there is exchange of materials between the biomimetic apoptotic bilayer and ANX-PS-Phy cubosomes, with an accumulation of ANX between the membrane and cubosomes possibly being the reason for the reduced cytotoxicity of ANX-PS-Phy cubosomes. A rat model of laser-induced choroidal neovascularization showed that ANX-PS-Phy cubosomes specifically targeted apoptotic cells in vivo. We propose that ANX-PS-Phy cubosomes are a potential candidate for ocular drug delivery for eye diseases.

Citation Format(s)

Annexin V-containing cubosomes for targeted early detection of apoptosis in degenerative retinal tissue. / Ding, Yue; Chow, Seong Hoong; Liu, Guei-Sheung; Wang, Bo; Lin, Tsung-Wu; Hsu, Hsien-Yi; Duff, Anthony P.; Le Brun, Anton P.; Shen, Hsin-Hui.

In: Journal of Materials Chemistry B, Vol. 6, No. 46, 14.12.2018, p. 7652-7661.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review