Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Yue Xu
  • Guive Balooch
  • Michael Chiou
  • Elena Bekerman
  • Michael T. Longaker

Detail(s)

Original languageEnglish
Pages (from-to)311-316
Journal / PublicationBiochemical and Biophysical Research Communications
Volume359
Issue number2
Online published25 May 2007
Publication statusPublished - 27 Jul 2007
Externally publishedYes

Abstract

Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitro model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation.

Research Area(s)

  • Cartilage repair, Chondrocyte, High density culture, Mechanical property, Mesenchymal stem cells, Nanoindentation, Tissue regeneration

Citation Format(s)

Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system. / Xu, Yue; Balooch, Guive; Chiou, Michael; Bekerman, Elena; Ritchie, Robert O.; Longaker, Michael T.

In: Biochemical and Biophysical Research Communications, Vol. 359, No. 2, 27.07.2007, p. 311-316.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review