TY - JOUR
T1 - Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system
AU - Xu, Yue
AU - Balooch, Guive
AU - Chiou, Michael
AU - Bekerman, Elena
AU - Ritchie, Robert O.
AU - Longaker, Michael T.
PY - 2007/7/27
Y1 - 2007/7/27
N2 - Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitro model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation.
AB - Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitro model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation.
KW - Cartilage repair
KW - Chondrocyte
KW - High density culture
KW - Mechanical property
KW - Mesenchymal stem cells
KW - Nanoindentation
KW - Tissue regeneration
UR - http://www.scopus.com/inward/record.url?scp=34249945668&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-34249945668&origin=recordpage
U2 - 10.1016/j.bbrc.2007.05.098
DO - 10.1016/j.bbrc.2007.05.098
M3 - RGC 21 - Publication in refereed journal
C2 - 17543281
SN - 0006-291X
VL - 359
SP - 311
EP - 316
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -