Anaerobic Oxidation of Methane Coupled with Dissimilatory Nitrate Reduction to Ammonium Fuels Anaerobic Ammonium Oxidation

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Wen-Bo Nie
  • Jie Ding
  • Guo-Jun Xie
  • Lu Yang
  • Lai Peng
  • Xin Tan
  • Bing-Feng Liu
  • De-Feng Xing
  • Nan-Qi Ren

Detail(s)

Original languageEnglish
Pages (from-to)1197-1208
Journal / PublicationEnvironmental Science and Technology
Volume55
Issue number2
Online published13 Nov 2020
Publication statusPublished - 19 Jan 2021
Externally publishedYes

Abstract

Nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) is critical for mitigating methane emission and returning reactive nitrogen to the atmosphere. The genomes of n-DAMO archaea show that they have the potential to couple anaerobic oxidation of methane to dissimilatory nitrate reduction to ammonium (DNRA). However, physiological details of DNRA for n-DAMO archaea were not reported yet. This work demonstrated n-DAMO archaea coupling the anaerobic oxidation of methane to DNRA, which fueled Anammox in a methane-fed membrane biofilm reactor with nitrate as only electron acceptor. Microelectrode analysis revealed that ammonium accumulated where nitrite built up in the biofilm. Ammonium production and significant upregulation of gene expression for DNRA were detected in suspended n-DAMO culture with nitrite exposure, indicating that nitrite triggered DNRA by n-DAMO archaea. 15N-labeling batch experiments revealed that n-DAMO archaea produced ammonium from nitrate rather than from external nitrite. Localized gradients of nitrite produced by n-DAMO archaea in biofilms induced ammonium production via the DNRA process, which promoted nitrite consumption by Anammox bacteria and in turn helped n-DAMO archaea resist stress from nitrite. As biofilms predominate in various ecosystems, anaerobic oxidation of methane coupled with DNRA could be an important link between the global carbon and nitrogen cycles that should be investigated in future research. © 2020 American Chemical Society.

Citation Format(s)

Anaerobic Oxidation of Methane Coupled with Dissimilatory Nitrate Reduction to Ammonium Fuels Anaerobic Ammonium Oxidation. / Nie, Wen-Bo; Ding, Jie; Xie, Guo-Jun et al.
In: Environmental Science and Technology, Vol. 55, No. 2, 19.01.2021, p. 1197-1208.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review