Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Adrian Oehmen
  • Raymond J. Zeng
  • Aaron M. Saunders
  • Linda L. Blackall
  • Jürg Keller

Detail(s)

Original languageEnglish
Pages (from-to)2767-2778
Journal / PublicationMicrobiology
Volume152
Issue number9
Publication statusPublished - Sept 2006
Externally publishedYes

Abstract

In the microbial competition observed in enhanced biological phosphorus removal (EBPR) systems, an undesirable group of micro-organisms known as glycogen-accumulating organisms (GAOs) compete for carbon in the anaerobic period with the desired polyphosphate-accumulating organisms (PAOs). Some studies have suggested that a propionate carbon source provides PAOs with a competitive advantage over GAOs in EBPR systems; however, the metabolism of GAOs with this carbon source has not been previously investigated. In this study, GAOs were enriched in a laboratory-scale bioreactor with propionate as the sole carbon source, in an effort to better understand their biochemical processes. Based on comprehensive solid-, liquid- and gas-phase chemical analytical data from the bioreactor, a metabolic model was proposed for the metabolism of propionate by GAOs. The model adequately described the anaerobic stoichiometry observed through chemical analysis, and can be a valuable tool for further investigation of the competition between PAOs and GAOs, and for the optimization of the EBPR process. A group of Alphaproteobacteria dominated the biomass (96% of Bacteria) from this bioreactor, while post-fluorescence in situ hybridization (FISH) chemical staining confirmed that these Alphaproteobacteria produced poly-β-hydroxyalkanoates (PHAs) anaerobically and utilized them aerobically, demonstrating that they were putative GAOs. Some of the Alphaproteobacteria were related to Defluvicoccus vanus (16% of Bacteria), but the specific identity of many could not be determined by FISH. Further investigation into the identity of other GAOs is necessary. © 2006 SGM.

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Citation Format(s)

Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source. / Oehmen, Adrian; Zeng, Raymond J.; Saunders, Aaron M. et al.
In: Microbiology, Vol. 152, No. 9, 09.2006, p. 2767-2778.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review