An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

6 Scopus Citations
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Author(s)

  • Meiling Piao
  • Pan Li
  • Xiaomin Zeng
  • Xi-Wen Wang
  • Lan Kang
  • Jinsong Zhang
  • Yifan Wei
  • Shaojun Zhang
  • Lei Tang
  • Jianghui Zhu
  • Xiaoyu Hu
  • Qiangfeng Cliff Zhang

Detail(s)

Original languageEnglish
Pages (from-to)2-13
Journal / PublicationFundamental Research
Volume2
Issue number1
Online published30 Dec 2021
Publication statusPublished - Jan 2022

Link(s)

Abstract

To enable diverse functions and precise regulation, an RNA sequence often folds into complex yet distinct structures in different cellular states. Probing RNA in its native environment is essential to uncovering RNA structures of biological contexts. However, current methods generally require large amounts of input RNA and are challenging for physiologically relevant use. Here, we report smartSHAPE, a new RNA structure probing method that requires very low amounts of RNA input due to the largely reduced artefact of probing signals and increased efficiency of library construction. Using smartSHAPE, we showcased the profiling of the RNA structure landscape of mouse intestinal macrophages upon inflammation, and provided evidence that RNA conformational changes regulate immune responses. These results demonstrate that smartSHAPE can greatly expand the scope of RNA structure-based investigations in practical biological systems, and also provide a research paradigm for the study of post-transcriptional regulation.

Research Area(s)

  • Low-input, Macrophage, RNA structure, RNA structure element, RNA structure probing method, RNA structurome

Citation Format(s)

An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages. / Piao, Meiling; Li, Pan; Zeng, Xiaomin et al.
In: Fundamental Research, Vol. 2, No. 1, 01.2022, p. 2-13.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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