An RNA G-Quadruplex Structure within the ADAR 5’UTR Interacts with DHX36 Helicase to Regulate Translation
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Article number | e202203553 |
Journal / Publication | Angewandte Chemie (International Edition) |
Volume | 61 |
Issue number | 52 |
Online published | 27 Oct 2022 |
Publication status | Published - 23 Dec 2022 |
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DOI | DOI |
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85143228026&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(ae4cff3a-d746-4302-a8af-f712152ae004).html |
Abstract
RNA G-quadruplex (rG4) structures in the 5′ untranslated region (5′UTR) play crucial roles in fundamental cellular processes. ADAR is an important enzyme that binds to double-strand RNA and accounts for the conversion of Adenosine to Inosine in RNA editing. However, so far there is no report on the formation and regulatory role of rG4 on ADAR expression. Here, we identify and characterize a thermostable rG4 structure within the 5′UTR of the ADAR1 mRNA and demonstrate its formation and inhibitory role on translation in reporter gene and native gene constructs. We reveal rG4-specific helicase DHX36 interacts with this rG4 in vitro and in cells under knockdown and knockout conditions by GTFH (G-quadruplex-triggered fluorogenic hybridization) probes and modulates translation in an rG4-dependent manner. Our results further substantiate the rG4 structure-DHX36 protein interaction in cells and highlight rG4 to be a key player in controlling ADAR1 translation.
Research Area(s)
- ADAR, DHX36, Gene Expression, RNA, G-Quadruplex, Structure-Function Relationship
Citation Format(s)
An RNA G-Quadruplex Structure within the ADAR 5’UTR Interacts with DHX36 Helicase to Regulate Translation. / Lyu, Kaixin; Chen, Shuo-Bin; Chow, Eugene Yui-Ching et al.
In: Angewandte Chemie (International Edition), Vol. 61, No. 52, e202203553, 23.12.2022.
In: Angewandte Chemie (International Edition), Vol. 61, No. 52, e202203553, 23.12.2022.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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