TY - JOUR
T1 - An Overview of Cellulase Immobilization Strategies for Biofuel Production
AU - Reshmy, R.
AU - Narisetty, Vivek
AU - Tarafdar, Ayon
AU - Bachan, Neena
AU - Madhavan, Aravind
AU - Tiwari, Archana
AU - Chaturvedi, Preeti
AU - Varjani, Sunita
AU - Sirohi, Ranjna
AU - Kumar, Vinod
AU - Awasthi, Mukesh Kumar
AU - Binod, Parameswaran
AU - Nagoth, Joseph Amruthraj
AU - Sindhu, Raveendran
PY - 2023/3
Y1 - 2023/3
N2 - The use of agricultural residue as a substrate and implementation of nanomaterials for cellulase immobilization can improve the efficiency at higher temperature and will lead to reduce the cost of cellulose-assisted biofuel production. The immobilization utilizing cellulase with amino, chitosan, or polymeric functionalities enhanced stability, activity, reusability, inhibition reduction, purification, and selectivity during enzymatic hydrolysis. Covalent interaction between the substrate and the cellulose marks in greater enzymatic immobilization, which pilots to higher biofuel production. Among the various techniques available for immobilization of cellulase on activated and functionalized magnetic nanoparticles, glutaraldehyde-based covalent binding are the most efficient process for cellulase immobilization. This review provides an overview of different cellulase immobilization strategies, factors, and its kinetics for enhanced biofuel production. The expanding need for low-cost immobilized cellulase, as well as its diverse applications in a variety of industries, is propelling research in this field. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
AB - The use of agricultural residue as a substrate and implementation of nanomaterials for cellulase immobilization can improve the efficiency at higher temperature and will lead to reduce the cost of cellulose-assisted biofuel production. The immobilization utilizing cellulase with amino, chitosan, or polymeric functionalities enhanced stability, activity, reusability, inhibition reduction, purification, and selectivity during enzymatic hydrolysis. Covalent interaction between the substrate and the cellulose marks in greater enzymatic immobilization, which pilots to higher biofuel production. Among the various techniques available for immobilization of cellulase on activated and functionalized magnetic nanoparticles, glutaraldehyde-based covalent binding are the most efficient process for cellulase immobilization. This review provides an overview of different cellulase immobilization strategies, factors, and its kinetics for enhanced biofuel production. The expanding need for low-cost immobilized cellulase, as well as its diverse applications in a variety of industries, is propelling research in this field. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
KW - Biofuel
KW - Cellulase
KW - Chitosan
KW - Covalent binding
KW - Enzymatic hydrolysis
KW - Enzyme immobilization
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85127530095&origin=recordpage
U2 - 10.1007/s12155-022-10431-3
DO - 10.1007/s12155-022-10431-3
M3 - RGC 21 - Publication in refereed journal
SN - 1939-1234
VL - 16
SP - 4
EP - 15
JO - Bioenergy Research
JF - Bioenergy Research
IS - 1
ER -
