An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria

P. Y. Liu; L. K. Chin; W. Ser; T. C. Ayi; P. H. Yap; T. Bourouina; Y. Leprince-Wang

doi:10.1039/c4lc00783b

An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria

P. Y. Liu
, L. K. Chin^*
, W. Ser^*
, T. C. Ayi
, P. H. Yap
, T. Bourouina
, Y. Leprince-Wang

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

68 Citations (Scopus)

Abstract

In this paper, for the first time, an on-chip optofluidic imaging system is innovated to measure the biophysical signatures of single waterborne bacteria, including both their refractive indices and morphologies (size and shape), based on immersion refractometry. The key features of the proposed optofluidic imaging platform include (1) multiple sites for single-bacterium trapping, which enable parallel measurements to achieve higher throughput, and (2) a chaotic micromixer, which enables efficient refractive index variation of the surrounding medium. In the experiments, the distinctive refractive index of Echerichia coli, Shigella flexneri and Vibrio cholera are measured with a high precision of 5 × 10^-3 RIU. The developed optofluidic imaging system has high potential not only for building up a database of biophysical signatures of waterborne bacteria, but also for developing single-bacterium detection in treated water that is in real-time, label-free and low cost. This journal is

Original language	English
Pages (from-to)	4237-4243
Journal	Lab on a Chip
Volume	14
Issue number	21
DOIs	https://doi.org/10.1039/c4lc00783b
Publication status	Published - 7 Nov 2014
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access Output

10.1039/c4lc00783b

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{b212ff5672cb45a49e8c6bb8e48315fe,

title = "An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria",

abstract = "In this paper, for the first time, an on-chip optofluidic imaging system is innovated to measure the biophysical signatures of single waterborne bacteria, including both their refractive indices and morphologies (size and shape), based on immersion refractometry. The key features of the proposed optofluidic imaging platform include (1) multiple sites for single-bacterium trapping, which enable parallel measurements to achieve higher throughput, and (2) a chaotic micromixer, which enables efficient refractive index variation of the surrounding medium. In the experiments, the distinctive refractive index of Echerichia coli, Shigella flexneri and Vibrio cholera are measured with a high precision of 5 × 10-3 RIU. The developed optofluidic imaging system has high potential not only for building up a database of biophysical signatures of waterborne bacteria, but also for developing single-bacterium detection in treated water that is in real-time, label-free and low cost. This journal is",

author = "Liu, \{P. Y.\} and Chin, \{L. K.\} and W. Ser and Ayi, \{T. C.\} and Yap, \{P. H.\} and T. Bourouina and Y. Leprince-Wang",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

year = "2014",

month = nov,

day = "7",

doi = "10.1039/c4lc00783b",

language = "English",

volume = "14",

pages = "4237--4243",

journal = "Lab on a Chip",

issn = "1473-0197",

publisher = "Royal Society of Chemistry",

number = "21",

}

TY - JOUR

T1 - An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria

AU - Liu, P. Y.

AU - Chin, L. K.

AU - Ser, W.

AU - Ayi, T. C.

AU - Yap, P. H.

AU - Bourouina, T.

AU - Leprince-Wang, Y.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2014/11/7

Y1 - 2014/11/7

N2 - In this paper, for the first time, an on-chip optofluidic imaging system is innovated to measure the biophysical signatures of single waterborne bacteria, including both their refractive indices and morphologies (size and shape), based on immersion refractometry. The key features of the proposed optofluidic imaging platform include (1) multiple sites for single-bacterium trapping, which enable parallel measurements to achieve higher throughput, and (2) a chaotic micromixer, which enables efficient refractive index variation of the surrounding medium. In the experiments, the distinctive refractive index of Echerichia coli, Shigella flexneri and Vibrio cholera are measured with a high precision of 5 × 10-3 RIU. The developed optofluidic imaging system has high potential not only for building up a database of biophysical signatures of waterborne bacteria, but also for developing single-bacterium detection in treated water that is in real-time, label-free and low cost. This journal is

AB - In this paper, for the first time, an on-chip optofluidic imaging system is innovated to measure the biophysical signatures of single waterborne bacteria, including both their refractive indices and morphologies (size and shape), based on immersion refractometry. The key features of the proposed optofluidic imaging platform include (1) multiple sites for single-bacterium trapping, which enable parallel measurements to achieve higher throughput, and (2) a chaotic micromixer, which enables efficient refractive index variation of the surrounding medium. In the experiments, the distinctive refractive index of Echerichia coli, Shigella flexneri and Vibrio cholera are measured with a high precision of 5 × 10-3 RIU. The developed optofluidic imaging system has high potential not only for building up a database of biophysical signatures of waterborne bacteria, but also for developing single-bacterium detection in treated water that is in real-time, label-free and low cost. This journal is

UR - https://www.scopus.com/pages/publications/84907658777

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84907658777&origin=recordpage

U2 - 10.1039/c4lc00783b

DO - 10.1039/c4lc00783b

M3 - RGC 21 - Publication in refereed journal

C2 - 25205636

SN - 1473-0197

VL - 14

SP - 4237

EP - 4243

JO - Lab on a Chip

JF - Lab on a Chip

IS - 21

ER -

An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria

Abstract

Bibliographical note

UN SDGs

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this