An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

Wen-Hsin Chang; Sung-Yi Yang; Chih-Hung Wang; Ping-Chen Li; Fuh-Jyh Jan; Tzong-Yueh Chen; Gwo-Bin Lee

doi:10.1109/NEMS.2012.6196757

An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

Wen-Hsin Chang
, Sung-Yi Yang
, Chih-Hung Wang
, Ping-Chen Li
, Fuh-Jyh Jan
, Tzong-Yueh Chen
, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system. © 2012 IEEE.

Original language	English
Title of host publication	2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages	204-207
DOIs	https://doi.org/10.1109/NEMS.2012.6196757
Publication status	Published - 2012
Externally published	Yes
Event	7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan Duration: 5 Mar 2012 → 8 Mar 2012

Publication series

Name	2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Conference

Conference	7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Place	Japan
City	Kyoto
Period	5/03/12 → 8/03/12

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

loop-mediated-isothermal-amplification (LAMP)
microfluidics
Phalaenopsis orchid

Access Output

10.1109/NEMS.2012.6196757

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

Chang, W.-H., Yang, S.-Y., Wang, C.-H., Li, P.-C., Jan, F.-J., Chen, T.-Y., & Lee, G.-B. (2012). An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 204-207). Article 6196757 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196757

Chang, Wen-Hsin ; Yang, Sung-Yi ; Wang, Chih-Hung et al. / An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. pp. 204-207 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

@inproceedings{df14e67757e9427c9bc4112bb0bd2637,

title = "An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids",

abstract = "Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system. {\textcopyright} 2012 IEEE.",

keywords = "loop-mediated-isothermal-amplification (LAMP), microfluidics, Phalaenopsis orchid",

author = "Wen-Hsin Chang and Sung-Yi Yang and Chih-Hung Wang and Ping-Chen Li and Fuh-Jyh Jan and Tzong-Yueh Chen and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 ; Conference date: 05-03-2012 Through 08-03-2012",

year = "2012",

doi = "10.1109/NEMS.2012.6196757",

language = "English",

isbn = "9781467311243",

series = "2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012",

pages = "204--207",

booktitle = "2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012",

}

Chang, W-H, Yang, S-Y, Wang, C-H, Li, P-C, Jan, F-J, Chen, T-Y & Lee, G-B 2012, An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. in 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012., 6196757, 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, pp. 204-207, 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, Kyoto, Japan, 5/03/12. https://doi.org/10.1109/NEMS.2012.6196757

An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. / Chang, Wen-Hsin; Yang, Sung-Yi; Wang, Chih-Hung et al.
2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 204-207 6196757 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

AU - Chang, Wen-Hsin

AU - Yang, Sung-Yi

AU - Wang, Chih-Hung

AU - Li, Ping-Chen

AU - Jan, Fuh-Jyh

AU - Chen, Tzong-Yueh

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2012

Y1 - 2012

N2 - Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system. © 2012 IEEE.

AB - Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system. © 2012 IEEE.

KW - loop-mediated-isothermal-amplification (LAMP)

KW - microfluidics

KW - Phalaenopsis orchid

UR - https://www.scopus.com/pages/publications/84861542459

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84861542459&origin=recordpage

U2 - 10.1109/NEMS.2012.6196757

DO - 10.1109/NEMS.2012.6196757

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781467311243

T3 - 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

SP - 204

EP - 207

BT - 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

T2 - 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Y2 - 5 March 2012 through 8 March 2012

ER -

Chang WH, Yang SY, Wang CH, Li PC, Jan FJ, Chen TY et al. An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 204-207. 6196757. (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). doi: 10.1109/NEMS.2012.6196757

An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this