An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

Ka-Ho Leung; Hong-Zhang He; Daniel Shiu-Hin Chan; Wai-Chung Fu; Chung-Hang Leung; Dik-Lung Ma

doi:10.1016/j.snb.2012.11.053

An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

Ka-Ho Leung
, Hong-Zhang He
, Daniel Shiu-Hin Chan
, Wai-Chung Fu
, Chung-Hang Leung
, Dik-Lung Ma^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

108 Citations (Scopus)

Abstract

We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution.

© 2012 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	487-492
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	177
Online published	27 Nov 2012
DOIs	https://doi.org/10.1016/j.snb.2012.11.053
Publication status	Published - Feb 2013
Externally published	Yes

Funding

This work is supported by Hong Kong Baptist University ( FRG2/11-12/009 ), Environment and Conservation Fund (ECF Project 3/2010), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund ( HMRF/11101212 ), the Research Grants Council ( HKBU/201811 and HKBU/204612 ), the Science and Technology Development Fund , Macao SAR ( 001/2012/A ) and the University of Macau ( SRG013-ICMS12-LCH , MYRG091(Y1-L2)-ICMS12-LCH and MYRG121(Y1-L2)-ICMS12-LCH ).

Research Keywords

Aptamer
Kanamycin
Luminescent
Platinum(II) complex
Switch-on probe

RGC Funding Information

RGC-funded

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title = "An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution",

abstract = "We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution. {\textcopyright} 2012 Elsevier B.V. All rights reserved.",

keywords = "Aptamer, Kanamycin, Luminescent, Platinum(II) complex, Switch-on probe",

author = "Ka-Ho Leung and Hong-Zhang He and Chan, \{Daniel Shiu-Hin\} and Wai-Chung Fu and Chung-Hang Leung and Dik-Lung Ma",

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doi = "10.1016/j.snb.2012.11.053",

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volume = "177",

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journal = "Sensors and Actuators, B: Chemical",

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TY - JOUR

T1 - An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

AU - Leung, Ka-Ho

AU - He, Hong-Zhang

AU - Chan, Daniel Shiu-Hin

AU - Fu, Wai-Chung

AU - Leung, Chung-Hang

AU - Ma, Dik-Lung

PY - 2013/2

Y1 - 2013/2

N2 - We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution. © 2012 Elsevier B.V. All rights reserved.

AB - We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution. © 2012 Elsevier B.V. All rights reserved.

KW - Aptamer

KW - Kanamycin

KW - Luminescent

KW - Platinum(II) complex

KW - Switch-on probe

UR - https://www.scopus.com/pages/publications/84871744180

UR - https://www.scopus.com/pages/publications/84871744180#tab=impact

U2 - 10.1016/j.snb.2012.11.053

DO - 10.1016/j.snb.2012.11.053

M3 - RGC 21 - Publication in refereed journal

AN - SCOPUS:84871744180

SN - 0925-4005

VL - 177

SP - 487

EP - 492

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

Abstract

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Research Keywords

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