An integrated microfluidic system with field-effect-transistor-based biosensors for automatic highly-sensitive C-reactive protein measurement
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Article number | 7051022 |
Pages (from-to) | 581-584 |
Journal / Publication | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
Volume | 2015-February |
Issue number | February |
Publication status | Published - 26 Feb 2015 |
Externally published | Yes |
Conference
Title | 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2015) |
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Location | Estoril Congress Center |
Place | Portugal |
City | Estoril |
Period | 18 - 22 January 2015 |
Link(s)
Abstract
Rapid and accurate diagnosis of C-reactive protein (CRP) is crucial for preventing cardiovascular diseases because it is a well-known biomarker for evaluating risks of cardiovascular diseases. Our previous work has shown that a microfluidic system equipped with a field-effect-transistor (FET)-based biosensor could detect CRP in 0.1X PBS and provided a limit of detection (LOD) of 26 pM CRP without gate bias. To improve the LOD, a new microfluidic device with a new methodology for measuring FET-based biosensors is presented in this study. Not only can the proposed system work in a solution with a physiological salt concentration but it also detects CRP with ultra-high sensitivity in an automatic fashion. This is the first time that a FET-based biosensor can effectively and automatically detect CRP in a physiological salt concentration without decreasing the sensitivity. The LOD of CRP using aptamer-immobilized AlGaN/GaN high-electron-mobility transistors (HEMTs) was experimentally found to be 1fM, demonstrating the superior performance of this new technique. It may be used as a point-of-care device for CRP detection in the near future.
Research Area(s)
Bibliographic Note
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Citation Format(s)
An integrated microfluidic system with field-effect-transistor-based biosensors for automatic highly-sensitive C-reactive protein measurement. / Chu, Chia-Ho; Chang, Wen-Hsin; Kao, Wei-Jer et al.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Vol. 2015-February, No. February, 7051022, 26.02.2015, p. 581-584.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Vol. 2015-February, No. February, 7051022, 26.02.2015, p. 581-584.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review