An integrated microfluidic system with field-effect-transistor-based biosensors for automatic highly-sensitive C-reactive protein measurement

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

4 Scopus Citations
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Author(s)

  • Chia-Ho Chu
  • Wen-Hsin Chang
  • Wei-Jer Kao
  • Chih-Lin Lin
  • Ko-Wei Chang
  • Yu-Lin Wang

Detail(s)

Original languageEnglish
Article number7051022
Pages (from-to)581-584
Journal / PublicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2015-February
Issue numberFebruary
Publication statusPublished - 26 Feb 2015
Externally publishedYes

Conference

Title28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2015)
LocationEstoril Congress Center
PlacePortugal
CityEstoril
Period18 - 22 January 2015

Abstract

Rapid and accurate diagnosis of C-reactive protein (CRP) is crucial for preventing cardiovascular diseases because it is a well-known biomarker for evaluating risks of cardiovascular diseases. Our previous work has shown that a microfluidic system equipped with a field-effect-transistor (FET)-based biosensor could detect CRP in 0.1X PBS and provided a limit of detection (LOD) of 26 pM CRP without gate bias. To improve the LOD, a new microfluidic device with a new methodology for measuring FET-based biosensors is presented in this study. Not only can the proposed system work in a solution with a physiological salt concentration but it also detects CRP with ultra-high sensitivity in an automatic fashion. This is the first time that a FET-based biosensor can effectively and automatically detect CRP in a physiological salt concentration without decreasing the sensitivity. The LOD of CRP using aptamer-immobilized AlGaN/GaN high-electron-mobility transistors (HEMTs) was experimentally found to be 1fM, demonstrating the superior performance of this new technique. It may be used as a point-of-care device for CRP detection in the near future.

Bibliographic Note

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Citation Format(s)

An integrated microfluidic system with field-effect-transistor-based biosensors for automatic highly-sensitive C-reactive protein measurement. / Chu, Chia-Ho; Chang, Wen-Hsin; Kao, Wei-Jer et al.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Vol. 2015-February, No. February, 7051022, 26.02.2015, p. 581-584.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review