AN integrated microfluidic system using field-effect transistors for CRP detection

Chih-Lin Lin; Yen-Wen Kang; Ko-Wei Chang; Wen-Hsin Chang; Yu-Lin Wang; Gwo-Bin Lee

doi:10.1109/MEMSYS.2014.6765622

AN integrated microfluidic system using field-effect transistors for CRP detection

Chih-Lin Lin
, Yen-Wen Kang
, Ko-Wei Chang
, Wen-Hsin Chang
, Yu-Lin Wang
, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

4 Citations (Scopus)

Abstract

Cardiovascular diseases are responsible for 25-million deaths worldwide on a yearly basis. Timely diagnosis of the disease is therefore an extensive research area. Toward this end, C-reactive protein (CRP) has become a reliable biomarker for evaluating risks of cardiovascular diseases. For commercial methods of CRP detection, the diagnosis of the enzyme-linked immunosorbent assay (ELISA) protocol and the high-sensitivity CRP (hs-CRP) were relatively time-consuming and the sensitivity and the detection limit of the above methods were not satisfactory. Recently, a CRP-specific aptamer (DNA-based) with high sensitivity and specificity was used to detect CRP in a microfluidic system, which was capable of performing the detection in an automated fashion while consuming tiny volumes of reagents and samples. Alternatively, AlGaN/GaN HEMT-based field-effect transistor (FET) sensors have emerged as promising biosensors to detect small molecules, proteins and even viruses, and have demonstrated rapid and highly sensitive detection in a compact system. To date, however, the combination of the microfluidic system, CRP-specific aptamer and high sensitivity FET sensors has not yet attempted. In this study, an integrated device that combined the advantages of microfluidics, CRP-specific aptamer and FET-based sensors was developed to achieve rapid, sensitive and specific CRP detection. © 2014 IEEE.

Original language	English
Title of host publication	MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	IEEE
Pages	250-253
ISBN (Print)	9781479935086
DOIs	https://doi.org/10.1109/MEMSYS.2014.6765622
Publication status	Published - 2014
Externally published	Yes
Event	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, United States Duration: 26 Jan 2014 → 30 Jan 2014 https://ieeexplore.ieee.org/xpl/conhome/6749560/proceeding

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Place	United States
City	San Francisco
Period	26/01/14 → 30/01/14
Internet address	https://ieeexplore.ieee.org/xpl/conhome/6749560/proceeding

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1109/MEMSYS.2014.6765622

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Cite this

Lin, C.-L., Kang, Y.-W., Chang, K.-W., Chang, W.-H., Wang, Y.-L., & Lee, G.-B. (2014). AN integrated microfluidic system using field-effect transistors for CRP detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 250-253). Article 6765622 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). IEEE. https://doi.org/10.1109/MEMSYS.2014.6765622

@inproceedings{401eac919a5948f29b1dddb8e6ff0562,

title = "AN integrated microfluidic system using field-effect transistors for CRP detection",

abstract = "Cardiovascular diseases are responsible for 25-million deaths worldwide on a yearly basis. Timely diagnosis of the disease is therefore an extensive research area. Toward this end, C-reactive protein (CRP) has become a reliable biomarker for evaluating risks of cardiovascular diseases. For commercial methods of CRP detection, the diagnosis of the enzyme-linked immunosorbent assay (ELISA) protocol and the high-sensitivity CRP (hs-CRP) were relatively time-consuming and the sensitivity and the detection limit of the above methods were not satisfactory. Recently, a CRP-specific aptamer (DNA-based) with high sensitivity and specificity was used to detect CRP in a microfluidic system, which was capable of performing the detection in an automated fashion while consuming tiny volumes of reagents and samples. Alternatively, AlGaN/GaN HEMT-based field-effect transistor (FET) sensors have emerged as promising biosensors to detect small molecules, proteins and even viruses, and have demonstrated rapid and highly sensitive detection in a compact system. To date, however, the combination of the microfluidic system, CRP-specific aptamer and high sensitivity FET sensors has not yet attempted. In this study, an integrated device that combined the advantages of microfluidics, CRP-specific aptamer and FET-based sensors was developed to achieve rapid, sensitive and specific CRP detection. {\textcopyright} 2014 IEEE.",

author = "Chih-Lin Lin and Yen-Wen Kang and Ko-Wei Chang and Wen-Hsin Chang and Yu-Lin Wang and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 ; Conference date: 26-01-2014 Through 30-01-2014",

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doi = "10.1109/MEMSYS.2014.6765622",

language = "English",

isbn = "9781479935086",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "IEEE",

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Lin, C-L, Kang, Y-W, Chang, K-W, Chang, W-H, Wang, Y-L & Lee, G-B 2014, AN integrated microfluidic system using field-effect transistors for CRP detection. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765622, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), IEEE, pp. 250-253, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, California, United States, 26/01/14. https://doi.org/10.1109/MEMSYS.2014.6765622

AN integrated microfluidic system using field-effect transistors for CRP detection. / Lin, Chih-Lin; Kang, Yen-Wen; Chang, Ko-Wei et al.
MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. IEEE, 2014. p. 250-253 6765622 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - AN integrated microfluidic system using field-effect transistors for CRP detection

AU - Lin, Chih-Lin

AU - Kang, Yen-Wen

AU - Chang, Ko-Wei

AU - Chang, Wen-Hsin

AU - Wang, Yu-Lin

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2014

Y1 - 2014

N2 - Cardiovascular diseases are responsible for 25-million deaths worldwide on a yearly basis. Timely diagnosis of the disease is therefore an extensive research area. Toward this end, C-reactive protein (CRP) has become a reliable biomarker for evaluating risks of cardiovascular diseases. For commercial methods of CRP detection, the diagnosis of the enzyme-linked immunosorbent assay (ELISA) protocol and the high-sensitivity CRP (hs-CRP) were relatively time-consuming and the sensitivity and the detection limit of the above methods were not satisfactory. Recently, a CRP-specific aptamer (DNA-based) with high sensitivity and specificity was used to detect CRP in a microfluidic system, which was capable of performing the detection in an automated fashion while consuming tiny volumes of reagents and samples. Alternatively, AlGaN/GaN HEMT-based field-effect transistor (FET) sensors have emerged as promising biosensors to detect small molecules, proteins and even viruses, and have demonstrated rapid and highly sensitive detection in a compact system. To date, however, the combination of the microfluidic system, CRP-specific aptamer and high sensitivity FET sensors has not yet attempted. In this study, an integrated device that combined the advantages of microfluidics, CRP-specific aptamer and FET-based sensors was developed to achieve rapid, sensitive and specific CRP detection. © 2014 IEEE.

AB - Cardiovascular diseases are responsible for 25-million deaths worldwide on a yearly basis. Timely diagnosis of the disease is therefore an extensive research area. Toward this end, C-reactive protein (CRP) has become a reliable biomarker for evaluating risks of cardiovascular diseases. For commercial methods of CRP detection, the diagnosis of the enzyme-linked immunosorbent assay (ELISA) protocol and the high-sensitivity CRP (hs-CRP) were relatively time-consuming and the sensitivity and the detection limit of the above methods were not satisfactory. Recently, a CRP-specific aptamer (DNA-based) with high sensitivity and specificity was used to detect CRP in a microfluidic system, which was capable of performing the detection in an automated fashion while consuming tiny volumes of reagents and samples. Alternatively, AlGaN/GaN HEMT-based field-effect transistor (FET) sensors have emerged as promising biosensors to detect small molecules, proteins and even viruses, and have demonstrated rapid and highly sensitive detection in a compact system. To date, however, the combination of the microfluidic system, CRP-specific aptamer and high sensitivity FET sensors has not yet attempted. In this study, an integrated device that combined the advantages of microfluidics, CRP-specific aptamer and FET-based sensors was developed to achieve rapid, sensitive and specific CRP detection. © 2014 IEEE.

UR - https://www.scopus.com/pages/publications/84899008490

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84899008490&origin=recordpage

U2 - 10.1109/MEMSYS.2014.6765622

DO - 10.1109/MEMSYS.2014.6765622

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781479935086

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 250

EP - 253

BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems

PB - IEEE

T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014

Y2 - 26 January 2014 through 30 January 2014

ER -

Lin CL, Kang YW, Chang KW, Chang WH, Wang YL, Lee GB. AN integrated microfluidic system using field-effect transistors for CRP detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. IEEE. 2014. p. 250-253. 6765622. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2014.6765622

AN integrated microfluidic system using field-effect transistors for CRP detection

Abstract

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Bibliographical note

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