TY - GEN
T1 - An integrated microfluidic system using buried optical fibers for detection of phalaenopsis orchid pathogens
AU - Lin, Chih-Lin
AU - Chang, Wen-Hsin
AU - Wang, Chih-Hung
AU - Li, Ping-Chen
AU - Jan, Fuh-Jyh
AU - Chen, Tzong-Yueh
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2013
Y1 - 2013
N2 - There is a critical need for early detection of pathogens in Phalaenopsis orchid due to its high economic value in agriculture. In our previous work [1], an integrated microfluidic system capable of detecting these pathogens through pathogen-specific ribonucleic acid (RNA) purification and RNA amplification by using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) was presented. In this study, a buried optical fiber-based detection module was integrated with the microfluidic chip to monitor the RT-LAMP reaction directly on-chip such that in-line optical detection can be realized. Successful RT-LAMP reaction, which amplifies the nucleic acids that yield the precipitate, magnesium pyrophosphate, and therefore increased the turbidity in the LAMP products could be detected optically. Furthermore, a fluorescent dye, calcein, could be also used in our system to detect the fluorescent signals, if necessary. Thus sensitive and fast diagnosis of the pathogens in Phalaenopsis orchids could be successfully achieved by using the integrated microfluidic system. © 2013 IEEE.
AB - There is a critical need for early detection of pathogens in Phalaenopsis orchid due to its high economic value in agriculture. In our previous work [1], an integrated microfluidic system capable of detecting these pathogens through pathogen-specific ribonucleic acid (RNA) purification and RNA amplification by using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) was presented. In this study, a buried optical fiber-based detection module was integrated with the microfluidic chip to monitor the RT-LAMP reaction directly on-chip such that in-line optical detection can be realized. Successful RT-LAMP reaction, which amplifies the nucleic acids that yield the precipitate, magnesium pyrophosphate, and therefore increased the turbidity in the LAMP products could be detected optically. Furthermore, a fluorescent dye, calcein, could be also used in our system to detect the fluorescent signals, if necessary. Thus sensitive and fast diagnosis of the pathogens in Phalaenopsis orchids could be successfully achieved by using the integrated microfluidic system. © 2013 IEEE.
KW - microfluidics
KW - optical fiber
KW - Reverse transcription Loop-medicated-isothermal-amplification (RT-LAMP)
UR - https://www.scopus.com/pages/publications/84891680135
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84891680135&origin=recordpage
U2 - 10.1109/Transducers.2013.6626997
DO - 10.1109/Transducers.2013.6626997
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781467359818
T3 - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
SP - 1231
EP - 1234
BT - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
T2 - 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS 2013)
Y2 - 16 June 2013 through 20 June 2013
ER -