TY - GEN
T1 - An integrated microfluidic system for diagnosis of quinolones resistance of helicobacter pylori
AU - Chao, Chih-Yu
AU - Wang, Chih-Hung
AU - Che, Yu-Jui
AU - Kao, Cheng-Yen
AU - Wu, Jiunn-Jong
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2014
Y1 - 2014
N2 - Helicobacter pylori (H. pylori) is a bacterium which can colonize the stomach mucosa and therefore play a crucial role in gastric diseases. Triple therapy treatment consisting of two antibiotics and a proton pump inhibitor has been routinely taken to eradicate H. pylori. Recently, some point mutations were found in gyrase genes against Quinolones. The highly frequent mutation sites were indicated as single amino acid substitution discovered in gyrase A subunit. The epsilometer test has been commonly used to confirm the antibiotic resistance after triple therapy treatment. However, the method is time-consuming and false negative results with trace amounts of H. pylori could be easily induced. Alternatively, conventional molecular diagnostic techniques such as polymer chain reaction (PCR) could be used to confirm the antibiotic resistance of H. pylori. However, this diagnostic process is relatively labor-intensive and requires expensive and bulky apparatus. In this study a new method was therefore developed to perform molecular diagnostic techniques of SNP-PCR on an integrated microfluidic system to detect the Quinolones resistance of H. pylori. © 2014 IEEE.
AB - Helicobacter pylori (H. pylori) is a bacterium which can colonize the stomach mucosa and therefore play a crucial role in gastric diseases. Triple therapy treatment consisting of two antibiotics and a proton pump inhibitor has been routinely taken to eradicate H. pylori. Recently, some point mutations were found in gyrase genes against Quinolones. The highly frequent mutation sites were indicated as single amino acid substitution discovered in gyrase A subunit. The epsilometer test has been commonly used to confirm the antibiotic resistance after triple therapy treatment. However, the method is time-consuming and false negative results with trace amounts of H. pylori could be easily induced. Alternatively, conventional molecular diagnostic techniques such as polymer chain reaction (PCR) could be used to confirm the antibiotic resistance of H. pylori. However, this diagnostic process is relatively labor-intensive and requires expensive and bulky apparatus. In this study a new method was therefore developed to perform molecular diagnostic techniques of SNP-PCR on an integrated microfluidic system to detect the Quinolones resistance of H. pylori. © 2014 IEEE.
UR - https://www.scopus.com/pages/publications/84898957382
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84898957382&origin=recordpage
U2 - 10.1109/MEMSYS.2014.6765767
DO - 10.1109/MEMSYS.2014.6765767
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781479935086
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 821
EP - 824
BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PB - IEEE
T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Y2 - 26 January 2014 through 30 January 2014
ER -