TY - GEN
T1 - An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process
AU - Gopinathan, Priya
AU - Hung, Lien-Yu
AU - Wang, Chih-Hung
AU - Chiang, Nai-Jung
AU - Wang, Yu-Chun
AU - Shan, Yan-Shen
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2016
Y1 - 2016
N2 - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.
AB - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.
KW - Aptamer
KW - Cell SELEX
KW - Cholangiocarcinoma
KW - Microfluidics
UR - https://www.scopus.com/pages/publications/85014147071
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85014147071&origin=recordpage
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9780979806490
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 930
EP - 931
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Y2 - 9 October 2016 through 13 October 2016
ER -