An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells

Wen-Yi Luo; Kuangwen Hsieh; Chien-Hsuan Tai; Gwo-Bin Lee

An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells

Wen-Yi Luo
, Kuangwen Hsieh
, Chien-Hsuan Tai
, Gwo-Bin Lee

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

Circulating tumor cells (CTCs) are indicators for cancer diagnosis and prognosis. In this work, a new integrated microfluidic system capable of un-biased and sensitive "negative" enrichment of CTCs was developed. By using anti-human CD45 coated magnetic beads, leukocytes were removed by external magnetic force, leaving behind an enriched target cell population. The results demonstrated that the developed chip was capable of performing CTC negative enrichment with comparable performance as the conventional benchtop method while offering significant advantages in rapid incubation and potential for automation, suggesting the developed chip is promising for standardized CTC isolation platform.

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	400-402
ISBN (Print)	9780979806476
Publication status	Published - 2014
Externally published	Yes
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014

Publication series

Name	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
Place	United States
City	San Antonio
Period	26/10/14 → 30/10/14

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Circulating tumor cells
Microfluidics
Negative enrichment

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Luo, W.-Y., Hsieh, K., Tai, C.-H., & Lee, G.-B. (2014). An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 400-402). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Luo, Wen-Yi ; Hsieh, Kuangwen ; Tai, Chien-Hsuan et al. / An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 400-402 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

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title = "An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells",

abstract = "Circulating tumor cells (CTCs) are indicators for cancer diagnosis and prognosis. In this work, a new integrated microfluidic system capable of un-biased and sensitive {"}negative{"} enrichment of CTCs was developed. By using anti-human CD45 coated magnetic beads, leukocytes were removed by external magnetic force, leaving behind an enriched target cell population. The results demonstrated that the developed chip was capable of performing CTC negative enrichment with comparable performance as the conventional benchtop method while offering significant advantages in rapid incubation and potential for automation, suggesting the developed chip is promising for standardized CTC isolation platform.",

keywords = "Circulating tumor cells, Microfluidics, Negative enrichment",

author = "Wen-Yi Luo and Kuangwen Hsieh and Chien-Hsuan Tai and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) ; Conference date: 26-10-2014 Through 30-10-2014",

year = "2014",

language = "English",

isbn = "9780979806476",

series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

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pages = "400--402",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

}

Luo, W-Y, Hsieh, K, Tai, C-H & Lee, G-B 2014, An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 400-402, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, Texas, United States, 26/10/14.

An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells. / Luo, Wen-Yi; Hsieh, Kuangwen; Tai, Chien-Hsuan et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 400-402 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells

AU - Luo, Wen-Yi

AU - Hsieh, Kuangwen

AU - Tai, Chien-Hsuan

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2014

Y1 - 2014

N2 - Circulating tumor cells (CTCs) are indicators for cancer diagnosis and prognosis. In this work, a new integrated microfluidic system capable of un-biased and sensitive "negative" enrichment of CTCs was developed. By using anti-human CD45 coated magnetic beads, leukocytes were removed by external magnetic force, leaving behind an enriched target cell population. The results demonstrated that the developed chip was capable of performing CTC negative enrichment with comparable performance as the conventional benchtop method while offering significant advantages in rapid incubation and potential for automation, suggesting the developed chip is promising for standardized CTC isolation platform.

AB - Circulating tumor cells (CTCs) are indicators for cancer diagnosis and prognosis. In this work, a new integrated microfluidic system capable of un-biased and sensitive "negative" enrichment of CTCs was developed. By using anti-human CD45 coated magnetic beads, leukocytes were removed by external magnetic force, leaving behind an enriched target cell population. The results demonstrated that the developed chip was capable of performing CTC negative enrichment with comparable performance as the conventional benchtop method while offering significant advantages in rapid incubation and potential for automation, suggesting the developed chip is promising for standardized CTC isolation platform.

KW - Circulating tumor cells

KW - Microfluidics

KW - Negative enrichment

UR - https://www.scopus.com/pages/publications/84941654163

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84941654163&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9780979806476

T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

SP - 400

EP - 402

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)

Y2 - 26 October 2014 through 30 October 2014

ER -

Luo WY, Hsieh K, Tai CH, Lee GB. An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 400-402. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

An integrated microfluidic platform for negative selection and enrichment of circulating tumor cells

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Other Access Options

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