An IncR Pplasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the blaKPC-2::NTEKPC-Id Fragment

Ning Dong; Lizhang Liu; Rong Zhang; Kaichao Chen; Miaomiao Xie; Edward Wai Chi Chan; Sheng Chen

doi:10.1128/AAC.01775-18

An IncR Pplasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the bla_KPC-2::NTE_KPC-Id Fragment

Ning Dong, Lizhang Liu, Rong Zhang, Kaichao Chen, Miaomiao Xie, Edward Wai Chi Chan, Sheng Chen^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

27 Citations (Scopus)

Abstract

Completed sequences of three plasmids from a carbapenem-resistant hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), the two multidrug-resistant plasmids (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) were predicted to originate from a single pKPC-CR-HvKP4-like multireplicon plasmid through homologous recombination. Interestingly, the bla_KPC-2 gene was detectable in five tandem repeats exhibiting the format of an NTE_KPC-Id-like transposon (IS26-ΔTn3-ISKpn8-bla_KPC-2-ΔISKpn6-korC-orf-IS26). The data suggest an important role of DNA recombination in mediating active plasmid evolution.

Original language	English
Article number	e01775-18
Journal	Antimicrobial Agents and Chemotherapy
Volume	63
Issue number	3
Online published	26 Feb 2019
DOIs	https://doi.org/10.1128/AAC.01775-18
Publication status	Published - Mar 2019
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

BlaKPC-2
Cointegration
Hypervirulent
Klebsiella pneumoniae
Tandem repeat

Access Output

10.1128/AAC.01775-18

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{3bb04368264640db88bd06a1f187286a,

title = "An IncR Pplasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the blaKPC-2::NTEKPC-Id Fragment",

abstract = "Completed sequences of three plasmids from a carbapenem-resistant hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), the two multidrug-resistant plasmids (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) were predicted to originate from a single pKPC-CR-HvKP4-like multireplicon plasmid through homologous recombination. Interestingly, the blaKPC-2 gene was detectable in five tandem repeats exhibiting the format of an NTEKPC-Id-like transposon (IS26-ΔTn3-ISKpn8-blaKPC-2-ΔISKpn6-korC-orf-IS26). The data suggest an important role of DNA recombination in mediating active plasmid evolution.",

keywords = "BlaKPC-2, Cointegration, Hypervirulent, Klebsiella pneumoniae, Tandem repeat",

author = "Ning Dong and Lizhang Liu and Rong Zhang and Kaichao Chen and Miaomiao Xie and Chan, {Edward Wai Chi} and Sheng Chen",

year = "2019",

month = mar,

doi = "10.1128/AAC.01775-18",

language = "English",

volume = "63",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "3",

}

An IncR Pplasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the bla_KPC-2::NTE_KPC-Id Fragment. / Dong, Ning; Liu, Lizhang; Zhang, Rong et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 63, No. 3, e01775-18, 03.2019.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - An IncR Pplasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the blaKPC-2::NTEKPC-Id Fragment

AU - Dong, Ning

AU - Liu, Lizhang

AU - Zhang, Rong

AU - Chen, Kaichao

AU - Xie, Miaomiao

AU - Chan, Edward Wai Chi

AU - Chen, Sheng

PY - 2019/3

Y1 - 2019/3

N2 - Completed sequences of three plasmids from a carbapenem-resistant hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), the two multidrug-resistant plasmids (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) were predicted to originate from a single pKPC-CR-HvKP4-like multireplicon plasmid through homologous recombination. Interestingly, the blaKPC-2 gene was detectable in five tandem repeats exhibiting the format of an NTEKPC-Id-like transposon (IS26-ΔTn3-ISKpn8-blaKPC-2-ΔISKpn6-korC-orf-IS26). The data suggest an important role of DNA recombination in mediating active plasmid evolution.

AB - Completed sequences of three plasmids from a carbapenem-resistant hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), the two multidrug-resistant plasmids (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) were predicted to originate from a single pKPC-CR-HvKP4-like multireplicon plasmid through homologous recombination. Interestingly, the blaKPC-2 gene was detectable in five tandem repeats exhibiting the format of an NTEKPC-Id-like transposon (IS26-ΔTn3-ISKpn8-blaKPC-2-ΔISKpn6-korC-orf-IS26). The data suggest an important role of DNA recombination in mediating active plasmid evolution.

KW - BlaKPC-2

KW - Cointegration

KW - Hypervirulent

KW - Klebsiella pneumoniae

KW - Tandem repeat

UR - http://www.scopus.com/inward/record.url?scp=85062288546&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85062288546&origin=recordpage

U2 - 10.1128/AAC.01775-18

DO - 10.1128/AAC.01775-18

M3 - RGC 21 - Publication in refereed journal

C2 - 30530604

SN - 0066-4804

VL - 63

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 3

M1 - e01775-18

ER -