An evanescent approach for mitochondrial function assay of living cells

De-Ming Yang; Chien-Chang Huang; Lung-Sen Kao; Chung-Chih Lin; Chin-Wen Chi; Hsia Yu Lin; Din Ping Tsai; Chen-Hsen Lee; Arthur Chiou

doi:10.1016/j.nano.2005.10.008

An evanescent approach for mitochondrial function assay of living cells

De-Ming Yang
, Chien-Chang Huang
, Lung-Sen Kao
, Chung-Chih Lin
, Chin-Wen Chi
, Hsia Yu Lin
, Din Ping Tsai
, Chen-Hsen Lee
, Arthur Chiou

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

5 Citations (Scopus)

Abstract

Mitochondria are now known to function physiologically not only in the production of ATP as the major cellular energy source, but also in the regulation of intracellular signaling, in, for example, stress-induced apoptosis and buffering of cytosolic calcium. It should be noted, when interpreting mitochondrial studies in situ, that mitochondria within cells show heterogeneity in both function and location. We applied both conventional epifluorescence microscopy (EPIFM) and total-internal-reflection fluorescence microscopy (TIRFM) in this study. Image data taken from TIRFM are excellent and markedly different from those taken from EPIFM. We further investigated the physiological variations of mitochondrial functions using an EPIFM/TIRFM dual-imaging system. This system permits further analysis of functions of mitochondria and other organelles with more precision than is possible using a traditional platform. © 2005.

Original language	English
Pages (from-to)	286-292
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	1
Issue number	4
DOIs	https://doi.org/10.1016/j.nano.2005.10.008
Publication status	Published - Dec 2005
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Evanescent wave
Mitochondria
Mitochondrial Ca2+
Pheochromocytoma PC12 cells
Total-internal-reflection fluorescence microscopy

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10.1016/j.nano.2005.10.008

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title = "An evanescent approach for mitochondrial function assay of living cells",

abstract = "Mitochondria are now known to function physiologically not only in the production of ATP as the major cellular energy source, but also in the regulation of intracellular signaling, in, for example, stress-induced apoptosis and buffering of cytosolic calcium. It should be noted, when interpreting mitochondrial studies in situ, that mitochondria within cells show heterogeneity in both function and location. We applied both conventional epifluorescence microscopy (EPIFM) and total-internal-reflection fluorescence microscopy (TIRFM) in this study. Image data taken from TIRFM are excellent and markedly different from those taken from EPIFM. We further investigated the physiological variations of mitochondrial functions using an EPIFM/TIRFM dual-imaging system. This system permits further analysis of functions of mitochondria and other organelles with more precision than is possible using a traditional platform. {\textcopyright} 2005.",

keywords = "Evanescent wave, Mitochondria, Mitochondrial Ca2+, Pheochromocytoma PC12 cells, Total-internal-reflection fluorescence microscopy",

author = "De-Ming Yang and Chien-Chang Huang and Lung-Sen Kao and Chung-Chih Lin and Chin-Wen Chi and Lin, \{Hsia Yu\} and Tsai, \{Din Ping\} and Chen-Hsen Lee and Arthur Chiou",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]. ",

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doi = "10.1016/j.nano.2005.10.008",

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TY - JOUR

T1 - An evanescent approach for mitochondrial function assay of living cells

AU - Yang, De-Ming

AU - Huang, Chien-Chang

AU - Kao, Lung-Sen

AU - Lin, Chung-Chih

AU - Chi, Chin-Wen

AU - Lin, Hsia Yu

AU - Tsai, Din Ping

AU - Lee, Chen-Hsen

AU - Chiou, Arthur

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2005/12

Y1 - 2005/12

N2 - Mitochondria are now known to function physiologically not only in the production of ATP as the major cellular energy source, but also in the regulation of intracellular signaling, in, for example, stress-induced apoptosis and buffering of cytosolic calcium. It should be noted, when interpreting mitochondrial studies in situ, that mitochondria within cells show heterogeneity in both function and location. We applied both conventional epifluorescence microscopy (EPIFM) and total-internal-reflection fluorescence microscopy (TIRFM) in this study. Image data taken from TIRFM are excellent and markedly different from those taken from EPIFM. We further investigated the physiological variations of mitochondrial functions using an EPIFM/TIRFM dual-imaging system. This system permits further analysis of functions of mitochondria and other organelles with more precision than is possible using a traditional platform. © 2005.

AB - Mitochondria are now known to function physiologically not only in the production of ATP as the major cellular energy source, but also in the regulation of intracellular signaling, in, for example, stress-induced apoptosis and buffering of cytosolic calcium. It should be noted, when interpreting mitochondrial studies in situ, that mitochondria within cells show heterogeneity in both function and location. We applied both conventional epifluorescence microscopy (EPIFM) and total-internal-reflection fluorescence microscopy (TIRFM) in this study. Image data taken from TIRFM are excellent and markedly different from those taken from EPIFM. We further investigated the physiological variations of mitochondrial functions using an EPIFM/TIRFM dual-imaging system. This system permits further analysis of functions of mitochondria and other organelles with more precision than is possible using a traditional platform. © 2005.

KW - Evanescent wave

KW - Mitochondria

KW - Mitochondrial Ca2+

KW - Pheochromocytoma PC12 cells

KW - Total-internal-reflection fluorescence microscopy

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U2 - 10.1016/j.nano.2005.10.008

DO - 10.1016/j.nano.2005.10.008

M3 - RGC 21 - Publication in refereed journal

C2 - 17292101

SN - 1549-9634

VL - 1

SP - 286

EP - 292

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

IS - 4

ER -

An evanescent approach for mitochondrial function assay of living cells

Abstract

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Research Keywords

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