An electrically-stimulate optically-record microsystem based on active CMOS multi-electrode array for dissociated cell cultures

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

  • Na Lei
  • Siddharth Ramakrishnan
  • Jason Orcutt
  • Lance Kam
  • Rafael Yuste
  • Ken Shepard

Detail(s)

Original languageEnglish
Title of host publicationProceedings of the 2011 IEEE/NIH Life Science Systems and Applications Workshop, LiSSA 2011
Pages163-166
Publication statusPublished - 2011
Externally publishedYes

Conference

Title2011 IEEE/NIH Life Science Systems and Applications Workshop, LiSSA 2011
PlaceUnited States
CityBethesda, MD
Period7 - 8 April 2011

Abstract

Calcium fluorescence-based optical recording combined with patch-clamp stimulation has become the standard technique for analyzing neural network behavior. At best, stimulation is limited to only a few channels in this case. Passive multielectrode arrays for two-dimensional electrophysiology only offer electrode densities of 60 electrodes per mm2. Here, we report an active multielectrode array, constructed with a standard complementary metal-oxide-semiconductor (CMOS) technology, to perform localized extracellular stimulation of dispersed cell cultures. A 256×256 array integrated with in-pixel stimulators on a 4-by-4 mm2 CMOS chip noninvasively stimulate hippocampal cells cultured on chip at cellular resolution. Combined with calcium imaging using high-affinity indicators, we demonstrate the ability to observe spatiotemporal dynamics of neural activity. © 2011 IEEE.

Citation Format(s)

An electrically-stimulate optically-record microsystem based on active CMOS multi-electrode array for dissociated cell cultures. / Lei, Na; Ramakrishnan, Siddharth; Shi, Peng; Orcutt, Jason; Kam, Lance; Yuste, Rafael; Shepard, Ken.

Proceedings of the 2011 IEEE/NIH Life Science Systems and Applications Workshop, LiSSA 2011. 2011. p. 163-166 5754184.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review