An array of 60,000 antibodies for proteome-scale antibody generation and target discovery

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

24 Scopus Citations
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Author(s)

  • Zhaohui Wang
  • Yang Li
  • Bing Hou
  • Mira I. Pronobis
  • Mingqiao Wang
  • Yuemeng Wang
  • Guangcun Cheng
  • Weining Weng
  • Yiqiang Wang
  • Yanfang Tang
  • Xuefan Xu
  • Rong Pan
  • Fei Lin
  • Nan Wang
  • Ziqing Chen
  • Shiwei Wang
  • Luyan Zulie Ma
  • Yangrui Li
  • Dongliang Huang
  • Li Jiang
  • Zhiqiang Wang
  • Wenfang Zeng
  • Ying Zhang
  • Xuemei Du
  • Ying Lin
  • Zhiqing Li
  • Qingyou Xia
  • Jing Geng
  • Huaping Dai
  • Yuan Yu
  • Xiao-Dong Zhao
  • Zheng Yuan
  • Qinghua Nie
  • Xiquan Zhang
  • Kun Wang
  • Fulin Chen
  • Qin Zhang
  • Yuxian Zhu
  • Susan Zheng
  • Kenneth D. Poss
  • Sheng-ce Tao
  • Xun Meng

Detail(s)

Original languageEnglish
Pages (from-to)eaax2271
Journal / PublicationScience Advances
Volume6
Issue number11
Online published11 Mar 2020
Publication statusPublished - Mar 2020
Externally publishedYes

Link(s)

Abstract

Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic antibody feature. Distinctive combinations of 10,000 to 20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-protein pairs were found for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for membrane proteins and chromatin immunoprecipitation-sequencing mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues identified internalizing tumor antigens for antibody-drug conjugates. By finding high-affinity mAbs at a fraction of current time and cost, PETAL enables proteome-scale antibody generation and target discovery.

Bibliographic Note

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

Citation Format(s)

An array of 60,000 antibodies for proteome-scale antibody generation and target discovery. / Wang, Zhaohui; Li, Yang; Hou, Bing et al.
In: Science Advances, Vol. 6, No. 11, 03.2020, p. eaax2271.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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