An Activatable NIR Probe for the Detection and Elimination of Senescent Cells
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
---|---|
Pages (from-to) | 5425–5431 |
Journal / Publication | Analytical Chemistry |
Volume | 94 |
Issue number | 13 |
Online published | 23 Mar 2022 |
Publication status | Published - 5 Apr 2022 |
Link(s)
Abstract
Cellular senescence is involved in diverse physiological processes. Accumulation of senescent cells can lead to numerous age-related diseases. Therefore, it is of great significance to develop chemical tools to effectively detect and eliminate senescent cells. Till date, a dual functional probe that could detect and eliminate senescent cells has yet been accomplished. Herein, a β-gal-activated probe, MB-βgal, based on the methylene blue (MB) fluorophore, was designed to detect and eliminate senescent cells. In the absence of β-gal, the probe showed no fluorescence and its 1O2 production efficiency was suppressed simultaneously. On the other hand, MB-βgal could be specifically activated by the high level of β-gal in senescent cells, thus, releasing free MB with near-infrared (NIR) fluorescence and high 1O2 production efficiency under light irradiation. MB-βgal demonstrated a fast response, high sensitivity, and high selectivity in detecting β-gal in an aqueous solution and was further applied to visualization and ablation of senescent cells. As a proof of concept, the dual functions of MB-βgal were successfully demonstrated in senescent HeLa cells and mouse embryonic fibroblast cells.
Citation Format(s)
An Activatable NIR Probe for the Detection and Elimination of Senescent Cells. / Yang, Liu; Liu, Guopan; Chen, Qingxin et al.
In: Analytical Chemistry, Vol. 94, No. 13, 05.04.2022, p. 5425–5431.
In: Analytical Chemistry, Vol. 94, No. 13, 05.04.2022, p. 5425–5431.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review