Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

22 Scopus Citations
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Author(s)

  • Zhijuan Wang
  • Juan Zhang
  • Changfeng Zhu
  • Shixin Wu
  • Daniel Mandler
  • Robert S. Marks

Detail(s)

Original languageEnglish
Pages (from-to)3110-3115
Journal / PublicationNanoscale
Volume6
Issue number6
Online published3 Jan 2014
Publication statusPublished - 21 Mar 2014
Externally publishedYes

Abstract

A sensitive electrochemical DNA biosensor based on the amplification of Au nanoparticles (AuNPs) has been developed. The AuNPs were modified with two types of signaling reporter DNAs, i.e. a methylene blue probe (MB-probe 2-SH) and T10 with a methylene blue signaling molecule (MB-T10-SH), forming DNA-AuNP conjugates. The MB-probe 2-SH is complementary to the target DNA, while MB-T10-SH is not. The presence of MB-T10-SH reduces the cross-reaction between target DNA and MB-probe 2-SH on the AuNPs, resulting in increased sensitivity of the biosensor. In our assay, the DNA sensor is fabricated by immobilizing a capture probe on the surface of the Au electrode, which then hybridizes with the corresponding target DNA, and further hybridizes with a DNA-Au conjugate. The signal of MB is measured by differential pulse voltammetry, while the DNA-Au conjugate enables the detection of target DNA in the linear range of 10-13 to 10-8 M with the detection limit as low as 50 fM. 

Citation Format(s)

Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA. / Wang, Zhijuan; Zhang, Juan; Zhu, Changfeng; Wu, Shixin; Mandler, Daniel; Marks, Robert S.; Zhang, Hua.

In: Nanoscale, Vol. 6, No. 6, 21.03.2014, p. 3110-3115.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review