All-Optical Modulation of Single Defects in Nanodiamonds: Revealing Rotational and Translational Motions in Cell Traction Force Fields

Lingzhi Wang; Yong Hou; Tongtong Zhang; Xi Wei; Yan Zhou; Dangyuan Lei; Qiang Wei; Yuan Lin; Zhiqin Chu

doi:10.1021/acs.nanolett.2c02232

All-Optical Modulation of Single Defects in Nanodiamonds: Revealing Rotational and Translational Motions in Cell Traction Force Fields

Lingzhi Wang, Yong Hou, Tongtong Zhang, Xi Wei, Yan Zhou, Dangyuan Lei, Qiang Wei, Yuan Lin^*, Zhiqin Chu^*

^*Corresponding author for this work

Department of Materials Science and Engineering

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

13 Citations (Scopus)

Abstract

Measuring the mechanical interplay between cells and their surrounding microenvironment is vital in cell biology and disease diagnosis. Most current methods can only capture the translational motion of fiduciary markers in the deformed matrix, but their rotational motions are normally ignored. Here, by utilizing single nitrogen-vacancy (NV) centers in nanodiamonds (NDs) as fluorescent markers, we propose a linear polarization modulation (LPM) method to monitor in-plane rotational and translational motions of the substrate caused by cell traction forces. Specifically, precise orientation measurement and localization with background suppression were achieved via optical polarization selective excitation of single NV centers with precisions of ∼0.5°/7.5 s and 2 nm/min, respectively. Additionally, we successfully applied this method to monitor the multidimensional movements of NDs attached to the vicinity of cell focal adhesions. The experimental results agreed well with our theoretical calculations, demonstrating the practicability of the NV-based LPM method in studying mechanobiology and cell-material interactions.

Original language	English
Pages (from-to)	7714–7723
Journal	Nano Letters
Volume	22
Issue number	18
Online published	10 Aug 2022
DOIs	https://doi.org/10.1021/acs.nanolett.2c02232
Publication status	Published - 28 Sept 2022

Funding

Z.Q.C. acknowledges the financial support from the HKSAR Research Grants Council (RGC) Early Career Scheme (ECS; No. 27202919); HKSAR RGC Research Matching Grant Scheme (RMGS; No. 207300313); HKSAR Innovation and Technology Fund (ITF) through the Platform Projects of the Innovation and Technology Support Program (ITSP; No. ITS/293/19FP); HKU Seed Fund (No. 202011159019 and No. 202010160007); and the Guangdong Special Support Project (No. 2019BT02 × 030). Y.L. acknowledges support by from the RGC General Research Fund (GRF; No. 17257016, No. 17210618, No. 17210520) and Health@InnoHK program of the Innovation and Technology Commission of the Hong Kong SAR Government, and the National Natural Science Foundation of China (No. 11872325). Y.Z. acknowledges the support by Guangdong Special Support Project (No. 2019BT02 × 030), Shenzhen Fundamental Research Fund (No. JCYJ20210324120213037). D.Y.L. acknowledges the financial support from the HKSAR RGC Areas of Excellence (AoE; No. AoE/P-701/20). All authors are grateful for Yayin Tan’s careful proof reading of this manuscript, and the general support from Xinhao Hu in some of the cell experiments.

Research Keywords

Cell traction force
Nanodiamonds
Nitrogen-vacancy centers
Polarization
Rotational motions

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title = "All-Optical Modulation of Single Defects in Nanodiamonds: Revealing Rotational and Translational Motions in Cell Traction Force Fields",

abstract = "Measuring the mechanical interplay between cells and their surrounding microenvironment is vital in cell biology and disease diagnosis. Most current methods can only capture the translational motion of fiduciary markers in the deformed matrix, but their rotational motions are normally ignored. Here, by utilizing single nitrogen-vacancy (NV) centers in nanodiamonds (NDs) as fluorescent markers, we propose a linear polarization modulation (LPM) method to monitor in-plane rotational and translational motions of the substrate caused by cell traction forces. Specifically, precise orientation measurement and localization with background suppression were achieved via optical polarization selective excitation of single NV centers with precisions of ∼0.5°/7.5 s and 2 nm/min, respectively. Additionally, we successfully applied this method to monitor the multidimensional movements of NDs attached to the vicinity of cell focal adhesions. The experimental results agreed well with our theoretical calculations, demonstrating the practicability of the NV-based LPM method in studying mechanobiology and cell-material interactions.",

keywords = "Cell traction force, Nanodiamonds, Nitrogen-vacancy centers, Polarization, Rotational motions",

author = "Lingzhi Wang and Yong Hou and Tongtong Zhang and Xi Wei and Yan Zhou and Dangyuan Lei and Qiang Wei and Yuan Lin and Zhiqin Chu",

year = "2022",

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T1 - All-Optical Modulation of Single Defects in Nanodiamonds

T2 - Revealing Rotational and Translational Motions in Cell Traction Force Fields

AU - Wang, Lingzhi

AU - Hou, Yong

AU - Zhang, Tongtong

AU - Wei, Xi

AU - Zhou, Yan

AU - Lei, Dangyuan

AU - Wei, Qiang

AU - Lin, Yuan

AU - Chu, Zhiqin

PY - 2022/9/28

Y1 - 2022/9/28

N2 - Measuring the mechanical interplay between cells and their surrounding microenvironment is vital in cell biology and disease diagnosis. Most current methods can only capture the translational motion of fiduciary markers in the deformed matrix, but their rotational motions are normally ignored. Here, by utilizing single nitrogen-vacancy (NV) centers in nanodiamonds (NDs) as fluorescent markers, we propose a linear polarization modulation (LPM) method to monitor in-plane rotational and translational motions of the substrate caused by cell traction forces. Specifically, precise orientation measurement and localization with background suppression were achieved via optical polarization selective excitation of single NV centers with precisions of ∼0.5°/7.5 s and 2 nm/min, respectively. Additionally, we successfully applied this method to monitor the multidimensional movements of NDs attached to the vicinity of cell focal adhesions. The experimental results agreed well with our theoretical calculations, demonstrating the practicability of the NV-based LPM method in studying mechanobiology and cell-material interactions.

AB - Measuring the mechanical interplay between cells and their surrounding microenvironment is vital in cell biology and disease diagnosis. Most current methods can only capture the translational motion of fiduciary markers in the deformed matrix, but their rotational motions are normally ignored. Here, by utilizing single nitrogen-vacancy (NV) centers in nanodiamonds (NDs) as fluorescent markers, we propose a linear polarization modulation (LPM) method to monitor in-plane rotational and translational motions of the substrate caused by cell traction forces. Specifically, precise orientation measurement and localization with background suppression were achieved via optical polarization selective excitation of single NV centers with precisions of ∼0.5°/7.5 s and 2 nm/min, respectively. Additionally, we successfully applied this method to monitor the multidimensional movements of NDs attached to the vicinity of cell focal adhesions. The experimental results agreed well with our theoretical calculations, demonstrating the practicability of the NV-based LPM method in studying mechanobiology and cell-material interactions.

KW - Cell traction force

KW - Nanodiamonds

KW - Nitrogen-vacancy centers

KW - Polarization

KW - Rotational motions

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DO - 10.1021/acs.nanolett.2c02232

M3 - RGC 21 - Publication in refereed journal

SN - 1530-6984

VL - 22

SP - 7714

EP - 7723

JO - Nano Letters

JF - Nano Letters

IS - 18

ER -

All-Optical Modulation of Single Defects in Nanodiamonds: Revealing Rotational and Translational Motions in Cell Traction Force Fields

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