AIEgen-deep: Deep learning of single AIEgen-imaging pattern for cancer cell discrimination and preclinical diagnosis

This study introduces AIEgen-Deep, an innovative classification program combining AIEgen fluorescent dyes, deep learning algorithms, and the Segment Anything Model (SAM) for accurate cancer cell identification. Our approach significantly reduces manual annotation efforts by 80%–90%. AIEgen-Deep demonstrates remarkable accuracy in recognizing cancer cell morphology, achieving a 75.9% accuracy rate across 26,693 images of eight different cell types. In binary classifications of healthy versus cancerous cells, it shows enhanced performance with an accuracy of 88.3% and a recall rate of 79.9%. The model effectively distinguishes between healthy cells (fibroblast and WBC) and various cancer cells (breast, bladder, and mesothelial), with accuracies of 89.0%, 88.6%, and 83.1%, respectively. Our method's broad applicability across different cancer types is anticipated to significantly contribute to early cancer detection and improve patient survival rates. © 2024 Elsevier B.V.