Advanced tools and methods for single-cell surgery

Adnan Shakoor; Wendi Gao; Libo Zhao; Zhuangde Jiang; Dong Sun

doi:10.1038/s41378-022-00376-0

Advanced tools and methods for single-cell surgery

Adnan Shakoor
, Wendi Gao
, Libo Zhao
, Zhuangde Jiang
, Dong Sun^*

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

74 Citations (Scopus)

160 Downloads (CityUHK Scholars)

Abstract

Highly precise micromanipulation tools that can manipulate and interrogate cell organelles and components must be developed to support the rapid development of new cell-based medical therapies, thereby facilitating in-depth understanding of cell dynamics, cell component functions, and disease mechanisms. This paper presents a literature review on micro/nanomanipulation tools and their control methods for single-cell surgery. Micromanipulation methods specifically based on laser, microneedle, and untethered micro/nanotools are presented in detail. The limitations of these techniques are also discussed. The biological significance and clinical applications of single-cell surgery are also addressed in this paper.

Original language	English
Article number	47
Journal	Microsystems & Nanoengineering
Volume	8
Online published	29 Apr 2022
DOIs	https://doi.org/10.1038/s41378-022-00376-0
Publication status	Published - 2022

Funding

This work was partly supported by grants from the Hong Kong Research Grants Council (Ref. nos. C1134-20G and 11211421), the Shenzhen Science and Technology Project, China (Ref. no. SGDX20201103093 00502), the National Science Foundation of China (Ref. no 52105589), and the RGC Postdoctoral Fellowship Scheme (PDFS) (Ref. no. PDFS2021-1S05).

Research Keywords

ATOMIC-FORCE MICROSCOPY
ROBOTIC SURGERY
CAPILLARY-ELECTROPHORESIS
MITOCHONDRIAL TRANSFER
OPTICAL MANIPULATION
IMAGE-RECONSTRUCTION
SILICON NANOWIRES
BIOLOGICAL CELLS
SYSTEM
DELIVERY

Publisher's Copyright Statement

This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

RGC Funding Information

RGC-funded

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10.1038/s41378-022-00376-0

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title = "Advanced tools and methods for single-cell surgery",

abstract = "Highly precise micromanipulation tools that can manipulate and interrogate cell organelles and components must be developed to support the rapid development of new cell-based medical therapies, thereby facilitating in-depth understanding of cell dynamics, cell component functions, and disease mechanisms. This paper presents a literature review on micro/nanomanipulation tools and their control methods for single-cell surgery. Micromanipulation methods specifically based on laser, microneedle, and untethered micro/nanotools are presented in detail. The limitations of these techniques are also discussed. The biological significance and clinical applications of single-cell surgery are also addressed in this paper.",

keywords = "ATOMIC-FORCE MICROSCOPY, ROBOTIC SURGERY, CAPILLARY-ELECTROPHORESIS, MITOCHONDRIAL TRANSFER, OPTICAL MANIPULATION, IMAGE-RECONSTRUCTION, SILICON NANOWIRES, BIOLOGICAL CELLS, SYSTEM, DELIVERY",

author = "Adnan Shakoor and Wendi Gao and Libo Zhao and Zhuangde Jiang and Dong Sun",

year = "2022",

doi = "10.1038/s41378-022-00376-0",

language = "English",

volume = "8",

journal = "Microsystems \& Nanoengineering",

issn = "2055-7434",

publisher = "Nature Publishing Group",

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TY - JOUR

T1 - Advanced tools and methods for single-cell surgery

AU - Shakoor, Adnan

AU - Gao, Wendi

AU - Zhao, Libo

AU - Jiang, Zhuangde

AU - Sun, Dong

PY - 2022

Y1 - 2022

N2 - Highly precise micromanipulation tools that can manipulate and interrogate cell organelles and components must be developed to support the rapid development of new cell-based medical therapies, thereby facilitating in-depth understanding of cell dynamics, cell component functions, and disease mechanisms. This paper presents a literature review on micro/nanomanipulation tools and their control methods for single-cell surgery. Micromanipulation methods specifically based on laser, microneedle, and untethered micro/nanotools are presented in detail. The limitations of these techniques are also discussed. The biological significance and clinical applications of single-cell surgery are also addressed in this paper.

AB - Highly precise micromanipulation tools that can manipulate and interrogate cell organelles and components must be developed to support the rapid development of new cell-based medical therapies, thereby facilitating in-depth understanding of cell dynamics, cell component functions, and disease mechanisms. This paper presents a literature review on micro/nanomanipulation tools and their control methods for single-cell surgery. Micromanipulation methods specifically based on laser, microneedle, and untethered micro/nanotools are presented in detail. The limitations of these techniques are also discussed. The biological significance and clinical applications of single-cell surgery are also addressed in this paper.

KW - ATOMIC-FORCE MICROSCOPY

KW - ROBOTIC SURGERY

KW - CAPILLARY-ELECTROPHORESIS

KW - MITOCHONDRIAL TRANSFER

KW - OPTICAL MANIPULATION

KW - IMAGE-RECONSTRUCTION

KW - SILICON NANOWIRES

KW - BIOLOGICAL CELLS

KW - SYSTEM

KW - DELIVERY

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U2 - 10.1038/s41378-022-00376-0

DO - 10.1038/s41378-022-00376-0

M3 - RGC 21 - Publication in refereed journal

C2 - 35502330

SN - 2055-7434

VL - 8

JO - Microsystems & Nanoengineering

JF - Microsystems & Nanoengineering

M1 - 47

ER -

Advanced tools and methods for single-cell surgery

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GRF: High-throughput Robotic Microinjection System and Its Application in Constructing Gene-edited Macrophages with Enhanced Tumor-killing Ability

CRF-Sub-pj: A Novel Vaccination Strategy: Using a Microrobot Platform for DNA Vaccine Delivery and Antigen Presentation

Cite this

Advanced tools and methods for single-cell surgery

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

Access Output

Other Access Options

Permanent Link

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Projects

GRF: High-throughput Robotic Microinjection System and Its Application in Constructing Gene-edited Macrophages with Enhanced Tumor-killing Ability

CRF-Sub-pj: A Novel Vaccination Strategy: Using a Microrobot Platform for DNA Vaccine Delivery and Antigen Presentation

Cite this