Adipose induces myoblast differentiation and mediates TNFα-regulated myogenesis

Yanling Wu; Qinqiang Long; Zifeng Zheng; Qiao Xia; Fenyun Wen; Xiaoyue Zhu; Xiaoling Yu; Zaiqing Yang

doi:10.1016/j.bbagrm.2014.07.018

Adipose induces myoblast differentiation and mediates TNFα-regulated myogenesis

Yanling Wu
, Qinqiang Long
, Zifeng Zheng
, Qiao Xia
, Fenyun Wen
, Xiaoyue Zhu
, Xiaoling Yu
, Zaiqing Yang

Centre of Super-Diamond and Advanced Films

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

5 Citations (Scopus)

Abstract

Skeletal muscle formation is controlled by multiple processes. These processes are tightly regulated by muscle regulatory factors. Genes that are highly and specifically expressed during myogenesis need to be identified. In the present study, the role of an anti-adipogenic gene adipose (Adp) in myogenesis is demonstrated. We discover that the expression of Adp is increased during myoblast differentiation. Overexpression of Adp in mouse myoblast C2C12 cells leads to an increase of myogenesis and up-regulation of MyoG expression. The inhibition effect of tumor necrosis factor α (TNFα) on myogenic differentiation is reversed by Adp-overexpression. Further research showed that TNFα significantly decreases Adp expression at both the mRNA and protein levels. Luciferase reporter assays showed that TNFα can inhibit Adp gene promoter activity and impair gene transcription. KLF15 was found to regulate the transcription of Adp. Furthermore, the expression of KLF15 and its binding to Adp promoter were reduced due to TNFα treatment. The reduced KLF15 expression after TNFα treatment is responsible for the repression of Adp gene promoter activity. KLF15 was also found to participate in Adp-mediated myogenic differentiation. Taken together, these data identify Adp as a positive modulator of myoblast differentiation and provide new insights for Adp function research. © 2014 Elsevier B.V.

Original language	English
Pages (from-to)	1183-1195
Journal	Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume	1839
Issue number	11
DOIs	https://doi.org/10.1016/j.bbagrm.2014.07.018
Publication status	Published - 1 Aug 2014

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

This work was supported by grants from the National Key Basic Research Program of China ( 2012CB124702 ), 948 Program ( 2012-S13 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110146130002 ), Program of National Natural Science Foundation of China ( 31172093 , 30970356 ), the National Science Foundation for Fostering Talents in Basic Research ( J1103510 ), and Huazhong Agricultural University Scientific and Technological Self-innovation Foundation ( 2013PY005 ).

Research Keywords

Adipose (Adp)
KLF15
Myogenesis
TNFα
Transcriptional regulation

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title = "Adipose induces myoblast differentiation and mediates TNFα-regulated myogenesis",

abstract = "Skeletal muscle formation is controlled by multiple processes. These processes are tightly regulated by muscle regulatory factors. Genes that are highly and specifically expressed during myogenesis need to be identified. In the present study, the role of an anti-adipogenic gene adipose (Adp) in myogenesis is demonstrated. We discover that the expression of Adp is increased during myoblast differentiation. Overexpression of Adp in mouse myoblast C2C12 cells leads to an increase of myogenesis and up-regulation of MyoG expression. The inhibition effect of tumor necrosis factor α (TNFα) on myogenic differentiation is reversed by Adp-overexpression. Further research showed that TNFα significantly decreases Adp expression at both the mRNA and protein levels. Luciferase reporter assays showed that TNFα can inhibit Adp gene promoter activity and impair gene transcription. KLF15 was found to regulate the transcription of Adp. Furthermore, the expression of KLF15 and its binding to Adp promoter were reduced due to TNFα treatment. The reduced KLF15 expression after TNFα treatment is responsible for the repression of Adp gene promoter activity. KLF15 was also found to participate in Adp-mediated myogenic differentiation. Taken together, these data identify Adp as a positive modulator of myoblast differentiation and provide new insights for Adp function research. {\textcopyright} 2014 Elsevier B.V.",

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author = "Yanling Wu and Qinqiang Long and Zifeng Zheng and Qiao Xia and Fenyun Wen and Xiaoyue Zhu and Xiaoling Yu and Zaiqing Yang",

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TY - JOUR

T1 - Adipose induces myoblast differentiation and mediates TNFα-regulated myogenesis

AU - Wu, Yanling

AU - Long, Qinqiang

AU - Zheng, Zifeng

AU - Xia, Qiao

AU - Wen, Fenyun

AU - Zhu, Xiaoyue

AU - Yu, Xiaoling

AU - Yang, Zaiqing

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Skeletal muscle formation is controlled by multiple processes. These processes are tightly regulated by muscle regulatory factors. Genes that are highly and specifically expressed during myogenesis need to be identified. In the present study, the role of an anti-adipogenic gene adipose (Adp) in myogenesis is demonstrated. We discover that the expression of Adp is increased during myoblast differentiation. Overexpression of Adp in mouse myoblast C2C12 cells leads to an increase of myogenesis and up-regulation of MyoG expression. The inhibition effect of tumor necrosis factor α (TNFα) on myogenic differentiation is reversed by Adp-overexpression. Further research showed that TNFα significantly decreases Adp expression at both the mRNA and protein levels. Luciferase reporter assays showed that TNFα can inhibit Adp gene promoter activity and impair gene transcription. KLF15 was found to regulate the transcription of Adp. Furthermore, the expression of KLF15 and its binding to Adp promoter were reduced due to TNFα treatment. The reduced KLF15 expression after TNFα treatment is responsible for the repression of Adp gene promoter activity. KLF15 was also found to participate in Adp-mediated myogenic differentiation. Taken together, these data identify Adp as a positive modulator of myoblast differentiation and provide new insights for Adp function research. © 2014 Elsevier B.V.

AB - Skeletal muscle formation is controlled by multiple processes. These processes are tightly regulated by muscle regulatory factors. Genes that are highly and specifically expressed during myogenesis need to be identified. In the present study, the role of an anti-adipogenic gene adipose (Adp) in myogenesis is demonstrated. We discover that the expression of Adp is increased during myoblast differentiation. Overexpression of Adp in mouse myoblast C2C12 cells leads to an increase of myogenesis and up-regulation of MyoG expression. The inhibition effect of tumor necrosis factor α (TNFα) on myogenic differentiation is reversed by Adp-overexpression. Further research showed that TNFα significantly decreases Adp expression at both the mRNA and protein levels. Luciferase reporter assays showed that TNFα can inhibit Adp gene promoter activity and impair gene transcription. KLF15 was found to regulate the transcription of Adp. Furthermore, the expression of KLF15 and its binding to Adp promoter were reduced due to TNFα treatment. The reduced KLF15 expression after TNFα treatment is responsible for the repression of Adp gene promoter activity. KLF15 was also found to participate in Adp-mediated myogenic differentiation. Taken together, these data identify Adp as a positive modulator of myoblast differentiation and provide new insights for Adp function research. © 2014 Elsevier B.V.

KW - Adipose (Adp)

KW - KLF15

KW - Myogenesis

KW - TNFα

KW - Transcriptional regulation

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SN - 1874-9399

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JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

JF - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

IS - 11

ER -

Adipose induces myoblast differentiation and mediates TNFα-regulated myogenesis

Abstract

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Research Keywords

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