Adenine deficient yeast : A fluorescent biosensor for the detection of Labile Zn(II) in aqueous solution

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Detail(s)

Original languageEnglish
Article number113075
Journal / PublicationBiosensors and Bioelectronics
Volume179
Online published6 Feb 2021
Publication statusPublished - 1 May 2021

Abstract

Labile Zn(II) species play key roles in inducing bioresponse. Thus the development of a biosensor for labile Zn(II) quantification is important. In this study, we demonstrate that the autofluorescence intensity (FITC channel) of an adenine deficient yeast (Ade(−) yeast) was enhanced in the presence of Zn2+. Yeast cells were firstly cultured for 24 h to obtain the Ade(−) yeast, and the biomass (OD value) was optimized to be 0.03. After pre-culturing in D-glucose at 2.5 g/L for 1 h, the cells were transferred to 2.5 g/L D-glucose containing Zn2+ and the autofluorescence intensity was determined by flow cytometry after 1 h. The biosensor could detect Zn2+ at ultralow concentration (0.01 μM) in the optimized medium and accurately quantify the extracellular concentrations of Zn2+ ranging from 0.01 to 0.5 μM. High tolerance of Ade(−) yeast to salinity, pH variation and other metals enabled its application as a biosensor for labile Zn detection in complex media. Determining dissolved Zn2+ from a viscous sample (zinc cream), Ade(−) yeast accurately quantified the labile Zn2+ with a lower quantification limit than the chemosensor and higher simplicity than the conventional method (ICP-MS coupled with ultrafiltration). The study provides a novel biosensor based on an Ade(−) yeast and could be potentially used to detect labile Zn(II) species at trace levels in complex media.

Research Area(s)

  • Autofluorescence, Biosensors, Labile Zn(II), Yeast