Active Manipulation of ECM Stiffness and Its Effect on Endothelial Cell Migration during Angiogenesis

Peter C.Y. Chen, Sahan C.B. Herath, Dong-an Wang, Harry Asada

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

6 Citations (Scopus)

Abstract

We have developed an engineering approach for actively manipulating the local stiffness of the extracellular matrix (ECM) and studied its effect on the migration of endothelial cells (ECs). In this approach, magnetic beads are embedded in an ECM through bio-conjugation between the Streptavidin-coated beads and the collagen fibers in order to alter the local stiffness of the ECM with the application of an external magnetic field. Our experimental results show that the magnetic forces acting on the embedded beads influence the behaviour of ECs during angiogenesis. These results suggest the possibility of creating desired stiffness gradients in an ECM for manipulating cell behavior in vitro.
Original languageEnglish
Title of host publicationProceedings of the World Congress on Engineering 2012
EditorsS. I. Ao, Len Gelman, David WL Hukins, Andrew Hunter, A. M. Korsunsky
PublisherNewswood Limited
Pages622-626
Volume1
ISBN (Electronic)9789881925138
Publication statusPublished - Jul 2012
Externally publishedYes
Event2012 World Congress on Engineering (WCE 2012) - London, United Kingdom
Duration: 4 Jul 20126 Jul 2012

Publication series

NameLecture Notes in Engineering and Computer Science
Volume2197
ISSN (Print)2078-0958
ISSN (Electronic)2078-0966

Conference

Conference2012 World Congress on Engineering (WCE 2012)
PlaceUnited Kingdom
CityLondon
Period4/07/126/07/12

Bibliographical note

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Research Keywords

  • Angiogenesis
  • Force
  • Magnetic field
  • Manipulation
  • Stiffness

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