TY - JOUR
T1 - Acoustic-fluorescence-activated-cell sorter for transfection cells screening
AU - Liu, Xianglian
AU - Shang, Guodong
AU - Zhang, Chuanyu
AU - Liu, Dan
AU - Zhang, Mingzhen
AU - Shum, Ho Cheung
AU - Wei, Xueyong
N1 - Publisher Copyright:
© 1964-2012 IEEE.
PY - 2024/12/9
Y1 - 2024/12/9
N2 - Biological particle sorting is a technology that isolates specific biological particles from complex samples based on their detectable characteristics. This technology has wide-ranging applications in disease diagnosis and treatment, drug development and evaluation, food safety, and environmental monitoring. Here we employed highly focused travelling surface acoustic waves combined with microfluidic chip as a strategy to solve the problem of biological damage and bioaerosol pollution, which can achieve high throughput separation of biological particles at low voltage. A fully functional acoustic FACS instrument consisting of fluorescence detection, sorting feedback, FTSAWs device and pressure control has been constructed. To verify its performance, we sorted fluorescent PS particles from nonfluorescent PS particles with the purity of 97.44±1.29% and the recovery of 96.15%. Further we employed the acoustic-fluorescence-activated sorter to screen transfection cells from initial cells. Fluorescent SGC-7901-GFP cells were sorted from nonfluorescent SGC-7901 cells with the purity of 91.67±1.91% and the recovery of 95.62%, and the viability of the post-sort cells was 97.78±1.01%. In addition, we have developed highly integrated portable equipment that is both low-cost and compact. This design effectively meets the requirements for equipment portability.© 2024 IEEE. Personal use is permitted, but republication/redistribution requires IEEE permission.
AB - Biological particle sorting is a technology that isolates specific biological particles from complex samples based on their detectable characteristics. This technology has wide-ranging applications in disease diagnosis and treatment, drug development and evaluation, food safety, and environmental monitoring. Here we employed highly focused travelling surface acoustic waves combined with microfluidic chip as a strategy to solve the problem of biological damage and bioaerosol pollution, which can achieve high throughput separation of biological particles at low voltage. A fully functional acoustic FACS instrument consisting of fluorescence detection, sorting feedback, FTSAWs device and pressure control has been constructed. To verify its performance, we sorted fluorescent PS particles from nonfluorescent PS particles with the purity of 97.44±1.29% and the recovery of 96.15%. Further we employed the acoustic-fluorescence-activated sorter to screen transfection cells from initial cells. Fluorescent SGC-7901-GFP cells were sorted from nonfluorescent SGC-7901 cells with the purity of 91.67±1.91% and the recovery of 95.62%, and the viability of the post-sort cells was 97.78±1.01%. In addition, we have developed highly integrated portable equipment that is both low-cost and compact. This design effectively meets the requirements for equipment portability.© 2024 IEEE. Personal use is permitted, but republication/redistribution requires IEEE permission.
KW - Microfluidic fluorescent activated cell sorters(FACS)
KW - Particle Sorting
KW - Portable Instrument
KW - Transfection cells
UR - http://www.scopus.com/inward/record.url?scp=85212183682&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85212183682&origin=recordpage
U2 - 10.1109/TBME.2024.3509522
DO - 10.1109/TBME.2024.3509522
M3 - RGC 21 - Publication in refereed journal
AN - SCOPUS:85212183682
SN - 0018-9294
JO - IEEE Transactions on Biomedical Engineering
JF - IEEE Transactions on Biomedical Engineering
ER -
