Abnormal scar identification with sphericalnucleic-acid technology

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

36 Scopus Citations
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Author(s)

  • David C. Yeo
  • Christian Wiraja
  • Amy S. Paller
  • Chad A. Mirkin
  • Chenjie Xu

Detail(s)

Original languageEnglish
Pages (from-to)227-238
Journal / PublicationNature Biomedical Engineering
Volume2
Issue number4
Online published13 Apr 2018
Publication statusPublished - Apr 2018
Externally publishedYes

Abstract

The accurate diagnosis of scar type and severity relies on histopathology of biopsied tissue, which is invasive and time-consuming, causes discomfort and may exacerbate scarring. Here, we show that imaging nanoprobes for the live-cell detection of intracellular messenger RNA (mRNA) (also known as NanoFlares) enable measurements of the expression of connective tissue growth factor (CTGF) as a visual indicator of hypertrophic scars and keloids. During cell culture, NanoFlares enabled the distinction of hypertrophic and keloidal fibroblasts from normal fibroblasts, and the detection of changes in CTGF expression resulting from the regulatory effects of transforming growth factor-β (TGF-β) agonists and TGF-β antagonists. We also applied the NanoFlares topically to the skin of live mice and rabbits, and to ex vivo human skin models. Transepidermal penetration of the NanoFlares enabled the visual and spectroscopic quantification of underlying abnormal fibroblasts on the basis of CTGF mRNA expression. Our proof-of-concept studies of topically applied NanoFlare technology as a means of biopsy-free scar diagnosis may eventually inform therapeutic decisions on the basis of the mRNA-expression patterns of skin disorders.

Citation Format(s)

Abnormal scar identification with sphericalnucleic-acid technology. / Yeo, David C.; Wiraja, Christian; Paller, Amy S.; Mirkin, Chad A.; Xu, Chenjie.

In: Nature Biomedical Engineering, Vol. 2, No. 4, 04.2018, p. 227-238.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review