A unidirectional cell switching gate by engineering grating length and bending angle

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Shu Fan Zhou
  • Singaram Gopalakrishnan
  • Jie Yang
  • Yun Wah Lam

Detail(s)

Original languageEnglish
Article numbere0147801
Journal / PublicationPLoS ONE
Volume11
Issue number1
Online published28 Jan 2016
Publication statusPublished - 2016

Link(s)

Abstract

On a microgrooved substrate, cells migrate along the pattern, and at random positions, reverse their directions. Here, we demonstrate that these reversals can be controlled by introducing discontinuities to the pattern. On "V-shaped grating patterns", mouse osteogenic progenitor MC3T3-E1 cells reversed predominately at the bends and the ends. The patterns were engineered in a way that the combined effects of angle- and length-dependence could be examined in addition to their individual effects. Results show that when the bend was placed closer to one end, migration behaviour of cells depends on their direction of approach. At an obtuse bend (135°), more cells reversed when approaching from the long segment than from the short segment. But at an acute bend (45°), this relationship was reversed. Based on this anisotropic behaviour, the designed patterns effectively allowed cells to move in one direction but blocked migrations in the opposing direction. This study demonstrates that by the strategic placement of bends and ends on grating patterns, we can engineer effective unidirectional switching gates that can control the movement of adherent cells. The knowledge developed in this study could be utilised in future cell sorting or filtering platforms without the need for chemotaxis or microfluidic control.

Research Area(s)

Citation Format(s)

A unidirectional cell switching gate by engineering grating length and bending angle. / Zhou, Shu Fan; Gopalakrishnan, Singaram; Xu, Yuan Hao et al.
In: PLoS ONE, Vol. 11, No. 1, e0147801, 2016.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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