A triphenylphosphonium-functionalised cyclometalated platinum(II) complex as a nucleolus-specific two-photon molecular dye

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chi-Kin Koo
  • Leo K.-Y. So
  • Ka-Leung Wong
  • Yu-Man Ho
  • Michael H.-W. Lam
  • Kwok-Wai Cheah
  • Chopen Chan-Wut Cheng
  • Wai-Ming Kwok

Detail(s)

Original languageEnglish
Pages (from-to)3942-3950
Journal / PublicationChemistry - A European Journal
Volume16
Issue number13
Publication statusPublished - 6 Apr 2010

Abstract

An organometallic cyclometalated platinum(II) complex, [Pt(L 3)Cl] [PF6], has been synthesised from a specially designed cyclometalating ligand, HL3 (triphenyl{5-[3-(6- phenylpyridin-2-yl)-l//-pyrazol-1-yl]pentyl}phosphonium chloride), that contains a pendant carbon chain carrying a terminal cationic triphenylphosphonium moiety. Aside from its room temperature single-photon luminescent properties in solution, [Pt(L3)Cl] + can also produce two-photon-induced luminescence at room temperature upon excitation at 700 nm from a mode- locked Ti: sapphire laser. Its twophoton absorption cross-section in DMF at room temperature was measured to be 28.0 x 10-50cm4 s photon-1. [Pt(L3)Cl]+ is able to selectively stain the cell nucleolus. This has been demonstrated by two-photon confocal imaging of live and methanol-fixed HeLa (human cervical carcinoma) and 3T3 (mouse skin fibroblasts) cells. This organelle specificity is likely to be related to its special affinity for proteins within cell nucleoli. As a result of such protein affinity, [Pt(L3)Cl]+ is an efficient RNA transcription inhibitor and shows rather profound cytotoxicity. On the other hand, the organelle-specific labelling and two-photon-induced luminescent properties of [Pt(L3)Cl]+ renders it a useful nuclear dye for the 3-dimensional reconstruction of optical sections of thick tissues, for example, mouse ileum tissues, by multiphoton confocal microscopy. © 2010 Wiley-VCH. Verlag GmbH & Co. KGaA.

Research Area(s)

  • Imaging agents, Optical tissue sectioning, Organelle-specific labeling, Platinum, Two-photon imaging

Citation Format(s)

A triphenylphosphonium-functionalised cyclometalated platinum(II) complex as a nucleolus-specific two-photon molecular dye. / Koo, Chi-Kin; So, Leo K.-Y.; Wong, Ka-Leung; Ho, Yu-Man; Lam, Yun-Wah; Lam, Michael H.-W.; Cheah, Kwok-Wai; Cheng, Chopen Chan-Wut; Kwok, Wai-Ming.

In: Chemistry - A European Journal, Vol. 16, No. 13, 06.04.2010, p. 3942-3950.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review