A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

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Author(s)

  • Peng Zhou
  • Becky K.C. Chan
  • Yuk Kei Wan
  • Chaya T.L. Yuen
  • Gigi C.G. Choi
  • Xinran Li
  • Cindy S.W. Tong
  • Sophia S.W. Zhong
  • Jieran Sun
  • Silvia Y.L. Mak
  • Maggie Z.Y. Chow
  • Jien Vei Khaw
  • Suet Yi Leung
  • Lydia W.T. Cheung
  • Kaeling Tan
  • Koon Ho Wong
  • H.Y. Edwin Chan
  • Alan S.L. Wong

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number108020
Journal / PublicationCell Reports
Volume32
Issue number6
Online published11 Aug 2020
Publication statusPublished - 11 Aug 2020

Link(s)

DOI

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Publisher's Copyright Statement
  • This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/
Link to Scopus https://www.scopus.com/record/display.uri?eid=2-s2.0-85089183838&origin=recordpage
Permanent Link https://scholars.cityu.edu.hk/en/publications/publication(8830a940-96db-456e-bbf7-2f4fe7bc7922).html

Abstract

We present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations and successfully validate double- and triple-combination regimens for suppression of cancer cell growth and protection against Parkinson's disease-associated toxicity. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets.

Research Area(s)

  • cancer, CombiGEM, combination therapy, combinatorial genetics, CRISPR, drug interaction, genetic interaction, high-throughput screening, neurodegeneration, Parkinson's disease

Citation Format(s)

[ RIS ] [ BibTeX ]

A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets. / Zhou, Peng; Chan, Becky K.C.; Wan, Yuk Kei et al.

In: Cell Reports, Vol. 32, No. 6, 108020, 11.08.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

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