A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
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Article number | 108020 |
Journal / Publication | Cell Reports |
Volume | 32 |
Issue number | 6 |
Online published | 11 Aug 2020 |
Publication status | Published - 11 Aug 2020 |
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DOI | DOI |
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Attachment(s) | Documents
Publisher's Copyright Statement
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85089183838&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(8830a940-96db-456e-bbf7-2f4fe7bc7922).html |
Abstract
We present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations and successfully validate double- and triple-combination regimens for suppression of cancer cell growth and protection against Parkinson's disease-associated toxicity. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets.
Research Area(s)
- cancer, CombiGEM, combination therapy, combinatorial genetics, CRISPR, drug interaction, genetic interaction, high-throughput screening, neurodegeneration, Parkinson's disease
Citation Format(s)
A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets. / Zhou, Peng; Chan, Becky K.C.; Wan, Yuk Kei et al.
In: Cell Reports, Vol. 32, No. 6, 108020, 11.08.2020.
In: Cell Reports, Vol. 32, No. 6, 108020, 11.08.2020.
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
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