A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Huiru Lu
  • Huatang Zhang
  • Jun Chen
  • Jinchao Zhang
  • Ruochuan Liu
  • Yuliang Zhao
  • Zhifang Chai
  • Yi Hu

Detail(s)

Original languageEnglish
Pages (from-to)477-482
Journal / PublicationTalanta
Volume146
Online published8 Sep 2015
Publication statusPublished - 1 Jan 2016

Abstract

In this study, Cu(II)-mediated differential alteration of cysteine (Cys) reactivity is reported by using a Cys-specific fluorescent probe. The probe could react with Cys to give out strong fluorescence. When Cys was preincubated with Cu(II), the fluorescence of the probe was decreased due to the inhibition of Cys's reactivity by Cu(II). Remarkably, experimental results reveal that the probe could detect Cu(II) at subnanomolar concentrations. In contrast, Cu(II) could only partially inhibit the reaction between Cys and Ellman's reagent (DTNB). Furthermore, selectivity experiments show that Cu(II) is a much more potent inhibitor for Cys compared to other metal ions. Cell imaging experiments also confirm the inhibitory effects of Cu(II) on Cys's reactivity in living cells. We envision that the probe could add a useful tool for sensitive and selective detection of Cu(II) for biomedical research.

Research Area(s)

  • Cell imaging, Copper, Cysteine, Ellman's reagent, Fluorescent probe

Citation Format(s)

A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II). / Lu, Huiru; Zhang, Huatang; Chen, Jun; Zhang, Jinchao; Liu, Ruochuan; Sun, Hongyan; Zhao, Yuliang; Chai, Zhifang; Hu, Yi.

In: Talanta, Vol. 146, 01.01.2016, p. 477-482.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review