A tetrahedral DNA nanorobot with conformational change in response to molecular trigger

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Fengyu Liu
  • Xiaoming Liu
  • Qing Shi
  • Christopher Maffeo
  • Masaru Kojima
  • Aleksei Aksimentiev
  • Qiang Huang
  • Toshio Fukuda
  • Tatsuo Arai

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)15552-15559
Journal / PublicationNanoscale
Volume13
Issue number37
Online published9 Aug 2021
Publication statusPublished - 7 Oct 2021

Abstract

Dynamic DNA origami nanostructures that respond to external stimuli are promising platforms for cargo delivery and nanoscale sensing. However, the low stability of such nanostructures under physiological conditions presents a major obstacle for their use in biomedical applications. This article describes a stable tetrahedral DNA nanorobot (TDN) programmed to undergo a controlled conformational change in response to epithelial cell adhesion molecule (EpCAM), a molecular biomarker specifically expressed on the circulating tumor cells. Multiresolution molecular dynamics simulations verified the overall stability of the folded TDN design and characterized local distortions in the folded structure. Atomic force microscopy and gel electrophoresis results showed that tetragonal structures are more stable than unfolded DNA origami sheets. Live cell experiments demonstrated the low cytotoxicity and target specificity of TDN. In summary, the proposed TDN can not only effectively resist nuclease catalysis but also has the potential to monitor EpCAM-positive cells precisely.

Research Area(s)

  • CELL ADHESION MOLECULE, FOLDING DNA, ORIGAMI, CANCER, NANOSTRUCTURES, DELIVERY, EXPRESSION, SELECTION, APTAMERS, SHAPES

Citation Format(s)

A tetrahedral DNA nanorobot with conformational change in response to molecular trigger. / Liu, Fengyu; Liu, Xiaoming; Shi, Qing; Maffeo, Christopher; Kojima, Masaru; Dong, Lixin; Aksimentiev, Aleksei; Huang, Qiang; Fukuda, Toshio; Arai, Tatsuo.

In: Nanoscale, Vol. 13, No. 37, 07.10.2021, p. 15552-15559.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review