A suction-type, pneumatic microfluidic device for rapid DNA extraction

Sung-Yi Yang; Chen-Hsun Weng; Gwo-Bin Lee

doi:10.1109/NEMS.2011.6017298

A suction-type, pneumatic microfluidic device for rapid DNA extraction

Sung-Yi Yang
, Chen-Hsun Weng
, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

3 Citations (Scopus)

Abstract

This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction. © 2011 IEEE.

Original language	English
Title of host publication	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages	71-74
DOIs	https://doi.org/10.1109/NEMS.2011.6017298
Publication status	Published - 2011
Externally published	Yes
Event	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan, China Duration: 20 Feb 2011 → 23 Feb 2011

Publication series

Name	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Conference

Conference	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Place	Taiwan, China
City	Kaohsiung
Period	20/02/11 → 23/02/11

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Research Keywords

CNC machining
DNA extraction
genomic DNA
MEMS
microfluidics
micromixer
micropump

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10.1109/NEMS.2011.6017298

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Yang, S.-Y., Weng, C.-H., & Lee, G.-B. (2011). A suction-type, pneumatic microfluidic device for rapid DNA extraction. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 71-74). Article 6017298 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017298

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title = "A suction-type, pneumatic microfluidic device for rapid DNA extraction",

abstract = "This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5\% can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction. {\textcopyright} 2011 IEEE.",

keywords = "CNC machining, DNA extraction, genomic DNA, MEMS, microfluidics, micromixer, micropump",

author = "Sung-Yi Yang and Chen-Hsun Weng and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",

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language = "English",

isbn = "9781612847757",

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Yang, S-Y, Weng, C-H & Lee, G-B 2011, A suction-type, pneumatic microfluidic device for rapid DNA extraction. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017298, NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, pp. 71-74, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, China, 20/02/11. https://doi.org/10.1109/NEMS.2011.6017298

A suction-type, pneumatic microfluidic device for rapid DNA extraction. / Yang, Sung-Yi; Weng, Chen-Hsun; Lee, Gwo-Bin.
NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 71-74 6017298 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - A suction-type, pneumatic microfluidic device for rapid DNA extraction

AU - Yang, Sung-Yi

AU - Weng, Chen-Hsun

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2011

Y1 - 2011

N2 - This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction. © 2011 IEEE.

AB - This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results showed the pumping rate can be as high as 400 μL/min at an applied pressure of -70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also showed that gDNA with an average concentration of 45 ± 3 ng/L and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a powerful platform for automatic, rapid DNA extraction. © 2011 IEEE.

KW - CNC machining

KW - DNA extraction

KW - genomic DNA

KW - MEMS

KW - microfluidics

KW - micromixer

KW - micropump

UR - https://www.scopus.com/pages/publications/80053339529

UR - https://www.scopus.com/pages/publications/80053339529#tab=impact

U2 - 10.1109/NEMS.2011.6017298

DO - 10.1109/NEMS.2011.6017298

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781612847757

T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

SP - 71

EP - 74

BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011

Y2 - 20 February 2011 through 23 February 2011

ER -

A suction-type, pneumatic microfluidic device for rapid DNA extraction

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Access Output

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