A stable RNA G-quadruplex within the 5′-UTR of Arabidopsis thaliana ATR mRNA inhibits translation

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chun Kit Kwok
  • Yiliang Ding
  • Saima Shahid
  • Sarah M. Assmann
  • Philip C. Bevilacqua

Detail(s)

Original languageEnglish
Pages (from-to)91-102
Journal / PublicationBiochemical Journal
Volume467
Issue number1
Publication statusPublished - 1 Apr 2015
Externally publishedYes

Abstract

Guanine quadruplex structures (GQSs) play important roles in the regulation of gene expression and cellular processes. Recent studies provide strong evidence for the formation and function of DNA and RNA GQSs in human cells. However, whether GQSs form and are functional in plants remains essentially unexplored. On the basis of circular dichroism (CD)-detected titration, UV-detected melting, in-line probing (ILP) and reporter gene assay studies, we report the first example of a plant RNA GQS that inhibits translation. This GQS is located within the 5′-UTR of the ATAXIA TELANGIECTASIA-MUTATED AND RAD3-RELATED (ATR) mRNA of Arabidopsis thaliana (mouse-ear cress). We show that this GQS is highly stable and is thermodynamically favoured over a competing hairpin structure in the 5′-UTR at physiological K+ and Mg2+ concentrations. Results from ILP reveal the secondary structure of the RNA and support formation of the GQS in vitro in the context of the complete 5′-UTR. Transient reporter gene assays performed in living plants reveal that the GQS inhibits translation but not transcription, implicating this GQS as a translational repressor in vivo. Our results provide the first complete demonstration of the formation and function of a regulatory RNA GQS in plants and open new avenues to explore potential functional roles of GQS in the plant kingdom.

Research Area(s)

  • 5′-untranslated region (5′-UTR), Arabidopsis thaliana, Ataxia Telangiectasia-mutated and RAD3-related (ATR), Guanine quadruplex structure (GQS), RNA structure, Translation

Citation Format(s)

A stable RNA G-quadruplex within the 5′-UTR of Arabidopsis thaliana ATR mRNA inhibits translation. / Kwok, Chun Kit; Ding, Yiliang; Shahid, Saima; Assmann, Sarah M.; Bevilacqua, Philip C.

In: Biochemical Journal, Vol. 467, No. 1, 01.04.2015, p. 91-102.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review