A Small RNA Transforms the Multidrug Resistance of Pseudomonas aeruginosa to Drug Susceptibility

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

4 Scopus Citations
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Author(s)

  • Carmen Oi Kwan Law
  • Qing Pan
  • Joseph Lee
  • Qin Hao
  • Ting-Fung Chan
  • Norman Wai Sing Lo
  • Irene Ling Ang
  • Alex Koon
  • Margaret Ip
  • Edwin Chan

Detail(s)

Original languageEnglish
Pages (from-to)218-228
Journal / PublicationMolecular Therapy - Nucleic Acids
Volume16
Online published22 Feb 2019
Publication statusPublished - 7 Jun 2019

Link(s)

Abstract

Bacteria with multiple drug resistance (MDR) have become a global issue worldwide, and hundreds of thousands of people's lives are threatened every year. The emergence of novel MDR strains and insufficient development of new antimicrobial agents are the major reasons that limit the choice of antibiotics for the treatment of bacterial infection. Thus, preserving the clinical value of current antibiotics could be one of the effective approaches to resolve this problem. Here we identified numerous novel small RNAs that were downregulated in the MDR clinical isolates of Pseudomonas aeruginosa (P. aeru), and we demonstrated that overexpression of one of these small RNAs (sRNAs), AS1974, was able to transform the MDR clinical strain to drug hypersusceptibility. AS1974 is the master regulator to moderate the expression of several drug resistance pathways, including membrane transporters and biofilm-associated antibiotic-resistant genes, and its expression is regulated by the methylation sites located at the 5′ UTR of the gene. Our findings unravel the sRNA that regulates the MDR pathways in clinical isolates of P. aeru. Moreover, transforming bacterial drug resistance to hypersusceptibility using sRNA could be the potential approach for tackling MDR bacteria in the future.

Research Area(s)

Citation Format(s)

A Small RNA Transforms the Multidrug Resistance of Pseudomonas aeruginosa to Drug Susceptibility. / Law, Carmen Oi Kwan; Huang, Chuan; Pan, Qing; Lee, Joseph; Hao, Qin; Chan, Ting-Fung; Lo, Norman Wai Sing; Ang, Irene Ling; Koon, Alex; Ip, Margaret; Chan, Edwin; Lau, Terrence Chi Kong.

In: Molecular Therapy - Nucleic Acids, Vol. 16, 07.06.2019, p. 218-228.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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