A simple method for making ion-selective microelectrodes suitable for intracellular recording in vertebrate cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)141-154
Journal / PublicationJournal of Neuroscience Methods
Volume15
Issue number2
Publication statusPublished - Oct 1985
Externally publishedYes

Abstract

A simple procedure for manufacturing Cl-, K+, and pH liquid membrane ion-sensitive microelectrodes is presented in detail. Electrodes suitable for recording from the specimen of interest are back-filled with a small amount of silane solution and heated for 5 min on a hot plate at a temperature between 400 and 500°C, after which they are injected with the ion-sensitive resin. The procedure is adaptable to many different glass stocks, e.g., single-barreled, double-barreled, or theta glass, and can be used to produce electrodes having a wide range of tip sizes for recording either extracellular or intracellular ion activities. Another advantage of the method is speed; up to 10 electrodes can be prepared simultaneously, permitting over 40 functional electrodes to be made per hour. © 1985.

Research Area(s)

  • extracellular ion activity, intracellular ion activity, ion activity, ion-sensitive microelectrode, silanization

Citation Format(s)