A self-driven carbon-doped high-density microwell array for single cell analysis

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Wenshuai Wu
  • Binh Thi Thanh Nguyen
  • Patricia Yang Liu
  • Gaozhe Cai
  • Shilun Feng
  • Yuzhi Shi
  • Boran Zhang
  • Yuzhi Hong
  • Ruozhen Yu
  • Xiaohong Zhou
  • Yi Zhang
  • Eric Peng Huat Yap
  • Ai Qun Liu

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number132198
Journal / PublicationSensors and Actuators B: Chemical
Volume368
Online published11 Jun 2022
Publication statusPublished - 1 Oct 2022

Abstract

Single cell analysis preserves the heterogeneity information of target cell population in search of rare biomarkers for disease diagnosis. Microfluidic technology facilitates single cell analysis through its high integrability with multi-functionalities, high sensitivity, precision and dynamic range for digital assays. However, demonstrated microfluidic devices for single cell analysis suffer from low throughput, the need of external instruments and complicated control system. Herein, we present a self-driven high-density microwell array for quantitative analysis of single-cell metabolic activity. 38,400-microwell array (density: 25,000/cm2) is achieved through two features: (1) Two-layered vertical design of microchannels to provide more space for microwell integration; and (2) Doping of carbon powder in microwell wall to block stray light transmission and improve signal-to-noise ratio, decreasing the interval between microwells down to 30 µm. Moreover, the chip is powered by pre-stored negative pressure without the need of external pump. Our microwell array significantly reduces the assay time from over 24–3 h in Escherichia coli quantitative analysis (6-order dynamic range). We also demonstrated the viability assay and metabolic heterogeneity of single bacteria, envisioning that the microwell array could be applied for other target cells and extended to different molecular techniques such as digital PCR.

Research Area(s)

  • High-throughput, Metabolic heterogeneity, Microfluidics, Single cell analysis, Viability assay

Citation Format(s)

A self-driven carbon-doped high-density microwell array for single cell analysis. / Wu, Wenshuai; Nguyen, Binh Thi Thanh; Liu, Patricia Yang; Cai, Gaozhe; Feng, Shilun; Shi, Yuzhi; Zhang, Boran; Hong, Yuzhi; Yu, Ruozhen; Zhou, Xiaohong; Zhang, Yi; Yap, Eric Peng Huat; Liu, Ai Qun; Chin, Lip Ket.

In: Sensors and Actuators B: Chemical, Vol. 368, 132198, 01.10.2022.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review